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目的观察地塞米松对大鼠肺缺血-再灌注(I-R)后肺细胞凋亡的影响。方法 40只健康的SD大鼠随机分为五组:假手术组(S)、单纯缺血组(I)、I-R组、小剂量地塞米松干预组(D1)及大剂量地塞米松干预组(D2),每组8只。根据Eppinger模型制作SD大鼠单侧肺I-R模型,HE染色观察组织病理学改变,TUNEL法检测肺细胞凋亡。结果 I-R组肺组织中肺细胞凋亡指数明显高于其它组(P<0.01)。D1、D2组肺细胞凋亡指数低于其它组,以D2组明显。病理切片显示I-R组肺组织结构损害分级显著重于其它组;D1、D2组肺组织结构损害分级下降,以D2组明显。结论 I-R可使肺细胞凋亡增加,地塞米松可以减少I-R后肺细胞凋亡,尤以大剂量明显。
Objective To observe the effect of dexamethasone on lung cell apoptosis after pulmonary ischemia-reperfusion (I-R) in rats. Methods Forty healthy SD rats were randomly divided into five groups: sham operation group (S), ischemia group (I), IR group, low dose dexamethasone intervention group (D1) and high dose dexamethasone intervention group (D2), 8 in each group. The I-R model of unilateral lung in SD rats was made according to the Eppinger model. The histopathological changes were observed by HE staining and the apoptosis of lung cells was detected by TUNEL. Results The lung cell apoptosis index in I-R group was significantly higher than that in other groups (P <0.01). The apoptosis index of lung cells in D1 and D2 groups was lower than that in other groups, especially in D2 group. The histopathological examination showed that the damage of lung tissue in I-R group was more serious than that in other groups. The damage of lung tissue in D1 and D2 group was reduced to D2 group. Conclusions I-R can increase the apoptosis of lung cells. Dexamethasone can reduce the apoptosis of lung cells after I-R, especially at high dose.