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为了保证紫花苜蓿扦插具有较高的成功率,试验采用土壤处理、根瘤菌接种、植物激素处理,研究这几个因素对紫花苜蓿扦插成活率的影响。结果表明:土壤处理能够显著影响紫花苜蓿扦插苗的成活率和生长,经过长时间阳光下消毒(温室内光照消毒30 d)的土壤紫花苜蓿扦插苗的成活率显著提高(P<0.05),高温长时间灭菌(150℃,10 h)的土壤则不利于扦插苗的生长;6-苄氨基嘌呤(6-BA)、2,4-二氯苯氧乙酸(2,4D)处理对紫花苜蓿扦插苗的生长有抑制作用;萘乙酸(NAA)和根瘤菌对紫花苜蓿扦插苗的影响相似,都能够提高紫花苜蓿扦插苗的成活率,提高扦插苗的长势和叶片数量。说明根瘤菌在侵染紫花苜蓿的时候产生了NAA或类似于NAA功能的促生长物质,从而促进插条生长。
In order to ensure a high success rate of alfalfa cuttings, soil treatment, rhizobia inoculation and plant hormone treatment were used to study the effects of these factors on the survival rate of alfalfa cutting. The results showed that soil treatment could significantly affect the survival rate and growth of cuttage seedlings of alfalfa. Survival rate of alfalfa cuttings after soaking in the sun for 30 days was significantly increased (P <0.05), high temperature The soil that was sterilized for a long time (150 ℃, 10 h) was not conducive to the growth of cutting seedlings; 6-benzylaminopurine (6-BA), 2,4-dichlorophenoxyacetic acid The growth of cutting seedlings was inhibited. NAA and rhizobium had similar effects on cuttings of alfalfa, which could increase the survival rate of cutting seedlings and increase the growth and leaf number of cutting seedlings. The result showed that rhizobia could promote the growth of cuttings by producing NAA or NAA-like growth-promoting substances when inoculating alfalfa.