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本研究应用免疫荧光结合逆行束路追踪技术观察了轴突切断对支配咬肌的三叉神经中脑核(Me5)神经元所含Trk受体蛋白,即TrkA、TrkB和TrkC表达的影响。通过大鼠咬肌神经给予荧光金,标记支配咬肌的Me5神经元;分别于切断咬肌神经后7和14 d对脑切片进行免疫组织化学染色并观察荧光金(FG)标记的Me5神经元表达的三种Trk受体。以双标记神经元占荧光金标记神经元总数的百分率为指标进行统计学分析,结果显示:(1)神经切断后7和14 d,TrkA免疫反应阳性神经元比例有显著性增加(P<0.05);(2)神经切断后随着存活时间的延长,TrkB免疫阳性神经元比例逐渐增加,但无显著性区别(P>0.05);(3)TrkC表达无显著性变化(P>0.05)。本研究结果提示,咬肌神经切断对三种Trk受体的表达有不同的影响,Trk受体表达的模式可能反应出Me5神经元对外周神经损伤后的一种适应。
In this study, immunofluorescence combined with retrograde beam-tracking technique was used to observe the effects of axotomy on the expression of Trk receptor, TrkA, TrkB and TrkC in the mesencephalon (Me5) neurons that innervate the masseter. Me5 neurons that dominate the masseter muscle were labeled with fluorescent gold through the masseter nerve in rats. Immunohistochemical staining of brain sections at 7 and 14 days after the masseter nerve was excised, respectively, and observation of FG-labeled Me5 neurons Three Trk receptors expressed. The results showed that: (1) The percentage of TrkA immunoreactive neurons increased significantly at 7 and 14 days after neurological incision (P <0.05), and the percentages of double labeled neurons in the total number of fluorescent gold labeled neurons were statistically analyzed. (2) The proportion of TrkB immunoreactive neurons increased gradually with the prolongation of survival time after neurotomy, but there was no significant difference (P> 0.05). (3) TrkC expression had no significant change (P> 0.05). Our results suggest that masseter nerve transection has different effects on the expression of three Trk receptors. The Trk receptor expression pattern may reflect the adaptation of Me5 neurons to peripheral nerve injury.