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目的 探讨 p16、p2 1蛋白和增殖细胞核抗原 (PCNA)在胃癌发生、发展中的作用及临床意义。方法 应用免疫组织化学法 ,对 46例胃癌及癌旁组织中p16、p2 1蛋白和PCNA进行检测。结果 胃癌组织中 p16、p2 1蛋白和PCNA的阳性率分别为 2 1.0 5 %、2 8.2 6%、73.91% ,与癌旁组织中的阳性率 5 8.70 %、65 .2 1%、2 3.91%差异有显著性 (P <0 .0 5 )。在胃癌的不同病理参数(淋巴结转移 ,分化程度 ,浸润深度及TNM分期 )中 ,p16,PCNA和 p2 1蛋白表达则分别在全部 4个 ,前 3个 ,前 2个参数中存在明显差异 (P <0 .0 5 )。p16、p2 1蛋白和PCNA在肿瘤的部位、大小、患者性别及年龄间差异无显著性 (P >0 .0 5 )。p16原癌基因蛋白质与PCNA ,p2 1与PCNA阳性表达存在明显差异 (P <0 .0 5 ) ,而p2 1表达与 p16表达之间则差异无显著性 ( P <0 .0 5 )。结论 p16、p2 1蛋白和PCNA是胃癌发生、发展过程中的重要因素 ,且与胃癌临床病理参数密切相关。
Objective To investigate the role and clinical significance of p16, p21 protein and proliferating cell nuclear antigen (PCNA) in the occurrence and development of gastric cancer. Methods Immunohistochemical method was used to detect the expression of p16, p2 1 protein and PCNA in 46 cases of gastric cancer and paracancerous tissues. Results The positive rates of p16, p2 1 protein and PCNA in gastric cancer tissue were 2 1.05%, 2 8.26% and 73.91%, respectively. The positive rates of p16, p21 and PCNA were 5.70%, 65.21% and 23.91% The difference was significant (P <0.05). The expression of p16, PCNA and p21 in different pathological parameters of gastric cancer (lymph node metastasis, differentiation degree, infiltration depth and TNM stage) were significantly different in all 4, first 3 and first 2 parameters (P <0 .0 5). There was no significant difference in the location, size, gender and age of the patients with p16, p2 1 protein and PCNA (P> 0.05). The positive expression of p16 protooncogene protein and PCNA, p2 1 and PCNA were significantly different (P <0.05), while there was no significant difference between p16 expression and p16 expression (P <0.05). Conclusion p16, p2 1 protein and PCNA are important factors in the development and progression of gastric cancer, and are closely related to the clinicopathological parameters of gastric cancer.