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目的研究宫颈癌与微卫星不稳定性的相关性,为探索宫颈癌的发病风险提供新的研究途径。方法采用PCR结合毛细管电泳的方法对常州地区120例宫颈癌患者(病例组)和102例健康女性(对照组)的外周静脉血样进行D1S1627、D22S1045基因座的基因型分析,并根据两组在D1S1627、D22S1045基因座的等位基因分布差异来推测与宫颈癌发生相关的易感因素和(或)抗性因素。结果病例组和对照组在D22S1045基因座上的等位基因频率分布差异存在统计学意义(P=0.008),在D1S1627基因座上的等位基因频率分布差异无统计学意义(P=0.095)。单因素分析显示两组在D22S1045基因座的等位基因14、17之间的频率分布差异均有统计学意义(P=0.026,P=0.014),OR值分别为3.837(95%CI:1.078～13.658)、0.520(95%CI:0.306～0.882)。多变量logistic回归分析显示产次、吸烟、D22S1045基因座等位基因14均为与宫颈癌发生相关的危险因素,OR值分别为2.063(95%CI:1.070～3.975)、1.958(95%CI:1.002～3.826)、2.766(95%CI:1.039～7.360)。结论 D22S1045基因座可能与宫颈癌的发生相关联,该基因座的等位基因14为宫颈癌的易感因素,等位基因17为宫颈癌的抗性因素。
Objective To study the correlation between cervical cancer and microsatellite instability and to provide a new approach to explore the risk of cervical cancer. Methods The peripheral blood samples from 120 patients with cervical cancer (case group) and 102 healthy women (control group) from Changzhou district were genotyped for D1S1627 and D22S1045 loci by PCR and capillary electrophoresis. According to the genotypes of D1S1627 and D22S1045 loci in two groups, , D22S1045 locus allelic distribution differences to speculate with the occurrence of cervical cancer-related susceptibility factors and (or) resistance factors. Results There were significant differences in allele frequency distribution between D22S1045 locus and control group (P = 0.008). There was no significant difference in allele frequency distribution between D1S1627 locus and control group (P = 0.095). Univariate analysis showed that there was significant difference in the frequency distribution between alleles 14 and 17 (P = 0.026, P = 0.014) in the two groups, with OR values of 3.837 (95% CI: 1.078 ~ 13.658), 0.520 (95% CI: 0.306 ~ 0.882). Multivariate logistic regression analysis showed that parity, smoking, and allele 14 of D22S1045 locus were all risk factors associated with cervical cancer. The OR values were 2.063 (95% CI: 1.070-3.975), 1.958 (95% CI: 1.002 ~ 3.826), 2.766 (95% CI: 1.039 ~ 7.360). Conclusion The D22S1045 locus may be associated with the occurrence of cervical cancer. The allele 14 of this locus is a susceptible factor for cervical cancer and the allele 17 is the resistance factor of cervical cancer.