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研究亚硝酸钠诱导活性氧对H22荷瘤小鼠肝癌细胞上皮间质转化和缺氧诱导因子1α(HIF-1α)的影响,并探讨HIF-1α在这一过程中的作用。制备H22肝癌细胞荷瘤小鼠模型,亚硝酸钠处理组每天分别灌胃给予10、20和30 mg.kg 1亚硝酸钠,对照组给予等体积生理盐水,连续给药21天。与对照组相比,亚硝酸钠处理组的移植瘤体积和重量没有明显变化,但是肿瘤组织内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性下降,亚硝酸盐和丙二醛(MDA)含量升高;癌细胞浸润周围的肌肉和筋膜;免疫组化和Western blotting结果显示,细胞波形蛋白和HIF-1α表达增强,E-钙粘素表达下降;肿瘤细胞呈现出上皮间质转化(EMT)。结果提示,亚硝酸钠在体内能够促进鼠肝癌细胞EMT发生,其机制与亚硝酸盐诱导活性氧(ROS)促进HIF-1α表达增加有关。
To investigate the effects of sodium nitrite on reactive oxygen species (ROS) and the expression of HIF-1α (HIF-1α) in H22 tumor-bearing mice and to investigate the role of HIF-1α in this process. H22 hepatocarcinoma tumor-bearing mice model was prepared. Sodium nitrite treatment groups were given intragastric administration of 10, 20 and 30 mg.kg 1 sodium nitrite respectively, while the control group was given equal volume of saline for consecutive 21 days. Compared with the control group, the volume and weight of the transplanted tumor in the sodium nitrite treatment group did not change significantly, but the activity of superoxide dismutase (SOD) and catalase (CAT) in the tumor tissue decreased. The nitrite and propane The content of MDA increased; cancer cells infiltrated the surrounding muscle and fascia; Immunohistochemistry and Western blotting showed that the expression of vimentin and HIF-1α was enhanced and the expression of E-cadherin was decreased; Interstitial Transformation (EMT). The results suggest that sodium nitrite can promote the EMT in murine hepatoma cells in vivo, and its mechanism is related to nitrite-induced reactive oxygen species (ROS) to promote the increase of HIF-1α expression.