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目的观察神经调节蛋白-1(NRG-1)对大鼠心室肌细胞L型钙电流(ICa-L)的影响。方法 C57小鼠取其心脏,经主动脉逆行插管后行Langendorff灌流,以酶解法分离获得单个心室肌细胞,采用全细胞膜片钳技术,记录NRG-1对L型钙电流(ICa-L)的影响。结果 0.05,0.1,0.2μmol/L NRG-1对ICa-L峰电流的抑制率分别为15.3%±2.2%,32.0%±4.7%,52.6%±6.1%(n=6,P<0.05),并使电流-电压曲线上移,激活延迟,失活后恢复时间延长。结论 NRG-1通过延迟钙通道的激活,延长失活后恢复时间,抑制心肌细胞ICa-L。
Objective To investigate the effect of neuregulin-1 (NRG-1) on L-type calcium current (ICa-L) in rat ventricular myocytes. Methods C57 mice were sacrificed and their hearts were perfused with Langendorff perfusion via a retrograde aortic cannulation. Single ventricular myocytes were isolated by enzymatic method. Whole cell patch clamp technique was used to record the effects of NRG-1 on L-type calcium current (ICa-L) Impact. Results The inhibitory rates of 0.05, 0.1 and 0.2 μmol / L NRG-1 on ICa-L peak currents were 15.3% ± 2.2%, 32.0% ± 4.7% and 52.6% ± 6.1% respectively (n = 6, And the current - voltage curve up, activation delay, recovery time after inactivation extended. Conclusion NRG-1 can delay the recovery of calcium channel and prolong the recovery time after inactivation, and inhibit ICa-L of cardiomyocytes.