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目的:建立苦豆子中苦豆碱的HPLC法;对苦豆子药材不同部位中苦豆碱含量进行考察,确定药材最佳采收部位。方法:采用Kromasil NH2(4.6 mm×250 mm,5μm)色谱柱,以乙腈-无水乙醇-3%磷酸水溶液(70∶15∶15)为流动相,检测波长205 nm,柱温25℃,流速1.0 mL.min-1。结果:苦豆碱的线性范围为11.21～224.20 mg.L-1(r=0.999 9),平均回收率(n=6)98.18%(RSD 4.1%);苦豆子叶、茎中苦豆碱含量分别为0.38%,0.02%,豆荚、花及种子中不含苦豆碱。结论:该方法简便、准确、重复性好,可以作为苦豆子中苦豆碱的含量测定方法;研究结果显示苦豆碱在叶子中含量最高,可以将叶子作为提取分离苦豆碱的最佳药材部位。
OBJECTIVE: To establish HPLC method for alperitine in Sophora alopecuroides L., and to investigate the content of alpericarb in different parts of Sophora alopecuroides to determine the optimum harvesting site. METHODS: Kromasil NH2 (4.6 mm × 250 mm, 5 μm) column was used with mobile phase of acetonitrile-absolute ethanol-3% phosphoric acid (70:15:15) as the mobile phase. The detection wavelength was set at 205 nm. 1.0 mL.min-1. Results: The linear range of alperitine was 11.21 ~ 224.20 mg.L-1 (r = 0.999 9), the average recovery was 98.18% (n = 6) Respectively, 0.38%, 0.02%, pods, flowers and seeds do not contain alginate. Conclusion: The method is simple, accurate and reproducible, and can be used as a method for the determination of content of alpericarb in Sophora alopecurica. The results showed that the content of alperitine in leaves is the highest, and the leaf can be used as the best medicine for extraction of alpericarb Site.