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我们采用脂质体介导方法,将外源性p16基因导入有其表达缺失的人胶质母细胞瘤细胞系BT325中,研究其对胶质母细胞瘤细胞周期、增殖及凋亡的影响,结果报道如下。一、材料和方法携带p16cDNA的真核表达载体pCDKN2WT由美国HJSuHuang教授惠赠?
We used liposome-mediated methods to introduce the exogenous p16 gene into the human glioblastoma cell line BT325 with its expression loss to study its cell cycle, proliferation and apoptosis of glioblastoma. Impact, the results are reported below. I. Materials and methods The eukaryotic expression vector pCDKN2WT carrying the p16 cDNA was gifted by Prof. H-JSu Huang of the United States.