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目的:建立反相高效液相色谱法同时测定蓝芩口服液中栀子苷、黄芩苷及盐酸小檗碱的含量。方法:采用Nova-PakC_(18)色谱柱(250mm×4.6mm,5μm),流动相组成为:A:乙腈,B:乙腈-0.5%三乙胺水溶液(磷酸调pH3.1)(15:85)梯度洗脱;流速1.0ml·min~(-1);检测波长254nm。结果:栀子苷、黄芩苷及盐酸小檗碱的线性范围分别为:0.5～2.5μg(r=0.9999),0.21～1.26μg(r=0.9994),0.05μg～1.59μg( r=0.9998)。平均回收率:栀子苷为100.8%,RSD=1.6%(n=9);黄芩苷为101.3%,RSD=2.0%(n=9)。盐酸小檗碱为100.0%,RSD=2.3%。(n=9)结论:方法简便,准确可作为蓝芩口服液的质量控制标准。
Objective: To establish a reversed-phase high performance liquid chromatography method for simultaneous determination of geniposide, baicalin and berberine hydrochloride in Lanqi Oral Liquid. METHODS: A Nova-Pak C 18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of: A: acetonitrile, B: acetonitrile-0.5% aqueous solution of triethylamine (pH adjusted to pH 3.1) (15:85) Gradient elution; flow rate 1.0 ml·min -1; detection wavelength 254 nm. RESULTS: The linear ranges of geniposide, baicalin and berberine hydrochloride were 0.5-2.5 μg (r=0.9999), 0.21-1.26 μg (r=0.9994), and 0.05 μg-1.59 μg (r=0.9998), respectively. The average recovery rate was: geniposide was 100.8%, RSD was 1.6% (n=9), baicalin was 101.3%, and RSD was 2.0% (n=9). Berberine hydrochloride was 100.0%, RSD=2.3%. (n=9) Conclusion: The method is simple and accurate. It can be used as the quality control standard of Lanjing Oral Liquid.