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目的:通过对动物模型的制作模拟新生儿围产期缺氧缺血性脑损伤,研究其脑组织病理变化,为新生儿缺氧缺血性脑损伤的病理生理的研究以及进一步有效的治疗提供实验基础。方法:将40只7 d新生昆明小鼠分四组,分别为正常组(A组)、单侧颈总动脉结扎组(B组)、单侧颈总动脉结扎+缺氧组(C组)和双侧颈总动脉结扎组(D组)。单侧颈总动脉结扎组(B组)行右侧颈总动脉结扎;单侧颈总动脉结扎+缺氧组(C组)行右侧颈总动脉结扎后将其置于20℃的恒温50 mL密闭容器中,分不同的时间将其取出;双侧颈总动脉结扎组(D组)行双侧颈总动脉结扎,各组术后均送回母鼠身边继续母乳喂养,三天后再作病理检测。结果:行单侧颈总动脉结扎加缺氧60 min时,小鼠结扎侧皮质及海马区出现病理改变,随着缺氧时间延长(90 min、100 min、120 min)病变范围逐渐扩大,病理改变越明显。结论:本实验显示单侧颈总动脉结扎同时缺氧一定时间可以导致小鼠脑组织损伤,脑细胞发生病理改变,且皮层及海马区域的神经细胞对缺氧缺血最为敏感,从而为进一步研究新生儿缺氧缺血性脑损伤提供了较为可靠的模型。
OBJECTIVE: To study the histopathological changes of neonatal hypoxic-ischemic brain damage induced by neonatal perinatal hypoxic-ischemic brain damage by making a model animal model for the study of the pathophysiology of neonatal hypoxic-ischemic brain damage and further effective treatment Experimental basis. Methods: Forty newborn Kunming mice were divided into four groups: normal group (group A), unilateral common carotid artery ligation group (group B), unilateral common carotid artery ligation + hypoxic group (group C) And bilateral carotid artery ligation group (D group). Unilateral common carotid artery ligation group (group B) underwent right common carotid artery ligation; unilateral common carotid artery ligation + hypoxic group (C group) right common carotid artery ligation after it was placed at 20 ℃ constant temperature 50 mL airtight container and removed at different times. Bilateral common carotid artery ligation group (group D) was ligated with bilateral common carotid artery. All the patients were returned to their mothers to continue breastfeeding, and three days later Pathological examination. Results: After unilateral carotid artery ligation plus hypoxia for 60 min, pathological changes occurred in the cortex and hippocampus of mice. With the prolongation of hypoxia time (90 min, 100 min, 120 min), the pathological changes The more obvious changes. Conclusion: This experiment shows that unilateral common carotid artery ligation while hypoxia for a certain period of time can lead to brain injury in mice, the pathological changes of brain cells, and cortical and hippocampal neurons in the most sensitive to hypoxic-ischemic, and thus for further study Neonatal hypoxic-ischemic brain injury provides a more reliable model.