论文部分内容阅读
利用数字化基因表达谱技术,对花后不同时期及不同环境温度条件下差异表达基因进行统计分析.将3份甘蓝型油菜于开花后20d移入到40℃高温和低温2个环境,分别处理12d和25d后取角果种子提取RNA,对花后不同时期及不同环境温度条件下差异表达基因进行统计分析.结果再以低温条件为对照,在高温下花后32d共检测出968个差异表达基因,其中上调表达基因为501个,下调表达基因为467个;在花后45d检测到1064个差异表达基因,其中上调表达基因为274个,下调表达基因为790个.结果表明,随着角果成熟,绝大多数基因的表达量减少,少数表达量显著上升的基因值得关注,它们可能与角果储藏物质的合成和角果成熟有关;在籽粒充实的高峰期(花后32d),高温胁迫下上调表达的基因较多,其中可能有一部分基因与环境应答有关.本研究结果与前人的结果在一定程度相似,表明本研究所采用的研究方法是可行的,获得的与环境胁迫相关基因为后续研究奠定了基础.
Statistical analysis was made on the differentially expressed genes at different post-anthesis stages and different ambient temperatures by using digital gene expression profiling.After 20 days of flowering, 3 parts of Brassica napus were transplanted to two environments of 40 ℃ in high temperature and low temperature for 12d and After 25 days, the anther seeds were taken for RNA extraction, and the differentially expressed genes at different post-anthesis periods and different ambient temperatures were statistically analyzed.Results A total of 968 differentially expressed genes were detected at 32 days after anthesis at low temperature, Among them, there were 501 up-regulated genes and 467 down-regulated genes, and 1064 differentially expressed genes were detected 45 days after anthesis, including 274 up-regulated genes and down-regulated genes of 790. The results showed that with the maturation of pods , Most of the gene expression decreased, a small number of genes with significant increase in expression of concern, which may be related to the synthesis of fruit storage materials and fruit maturity; in the peak of grain filling (32d after flowering), under high temperature stress There are many genes that are up-regulated, some of which may be related to environmental responses.The results of this study are similar to those of our predecessors to a certain degree, indicating that the genes used in this study The research method is feasible. The acquired genes related to environmental stress lay the foundation for the follow-up study.