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目的:研究TGF-β1是否能在人母-胎界面诱导生成T调节性(Treg)细胞。方法:早孕妇女外周血和蜕膜CD4+CD25-T细胞中加入不同浓度的TGF-β1(0 ng/ml、2 ng/ml、5 ng/ml、10 ng/ml)分别于培养后第2日、第4日和第6日用流式细胞仪检测培养Foxp3的表达情况,随后将诱导生成的CD4+Foxp3+T细胞与CD8+T细胞混合培养,观察后者凋亡因子CD95配体(CD95L)的表达情况。结果:体外培养中,TGF-β1可诱导早孕妇女的外周血和蜕膜CD4+CD25-T细胞生成诱导性Treg细胞,随着培养时间增加而增强诱导效应,且在TGF-β1浓度为5 ng/ml时诱导功能最强;诱导生成的CD4+Foxp3+T细胞具有促进效应细胞CD8+T细胞凋亡的功能。结论:体外培养中,TGF-β1能将人外周血和蜕膜诱导生成Treg细胞,且具有免疫抑制功能,有良好的免疫治疗前景。
AIM: To investigate whether TGF-β1 induces T-regulatory (Treg) cells at the maternal-fetal interface. Methods: Different concentrations of TGF-β1 (0 ng / ml, 2 ng / ml, 5 ng / ml and 10 ng / ml) were added into peripheral blood and deciduas CD4 Day, day 4 and day 6, the expression of Foxp3 was detected by flow cytometry. Subsequently, the induced CD4 + Foxp3 + T cells were mixed with CD8 + T cells to observe the expression of the latter apoptotic factor CD95 ligand CD95L) expression. Results: In vitro, TGF-β1 induced the induction of Treg cells from peripheral blood and decidual CD4 + CD25-T cells in early pregnant women, and enhanced the induction of Treg cells with the increase of incubation time. TGF-β1 concentration was 5 ng / ml when the induction of the strongest; induced CD4 + Foxp3 + T cells have the role of promoting effector cells CD8 + T cell apoptosis. CONCLUSION: In vitro culture, TGF-β1 can induce the generation of Treg cells in human peripheral blood and decidua, and has immunosuppressive function and good immunotherapy prospect.