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为了对小麦—黑麦１染色体易位系进行一般性鉴别和特殊性分析，分离单克隆抗体（ｍＡｂ），分别用于识别在１Ｂ染色体编码的Ｍｒ４００００γ—黑麦碱（８０８／１０）和醇溶蛋白（２１８／７）．用２—丙醇水溶液对面粉、粗粉或半籽粒进行提取．并直接进行酶联免疫吸附法（ＥＬＩＳＡ）分析，分析表明，当ＩＲＳ与１Ａ、１Ｂ和１Ｄ发生易位时，ｍＡｂ８０８／１０表现出阳性反应，而非易位小麦反应很弱．抗体ｍＡｂ８０８／１０对黑麦碱的束缚决定于能保持三维结构的蛋白质，如果蛋白质降解，抗体反应就会消失．ｍＡｂ２１８／１７几乎对１ＢＬ．１ＲＳ易位系的２—丙醇提取物不起反应，但对其它被测小麦呈阳性颜色反应．因此，１ＢＬ．１ＲＳ易位系的检测能够通过ｍＡｂ８０８／１０的阳性反应或ｍＡｂ２１８／１７的阴性反应来完成．此外，对单个２—丙醇样品提取物进行两项ＥＬＩＳＡ操作，能够鉴别出三个群体：１ＢＬ．１ＲＳ易位系、１ＡＬ．１ＲＳ易位系和非易位小麦．这些ＥＬＩＳＡ分析方法较之其它Ｇｌｉ—Ｂ１醇溶蛋白或Ｓｅｃ—１黑麦碱的免疫分析法具有简化步骤，缩短时间，样品筛选率高和可适用于面粉、粗粉或半籽粒样品的优点．
For general identification and specificity analysis of wheat-rye 1 translocation lines, monoclonal antibodies (mAb) were isolated and used to identify the Mr40000γ-rye (808/10) and alcohol-soluble Protein (218/7). Flour, semolina or semi-grain is extracted with 2-propanol in water. The results of ELISA showed that mAb808 / 10 showed a positive reaction when translocating IRS to 1A, 1B and 1D, but the reaction of non-translocating wheat was weak. Antibody mAb808 / 10 bound to the rye base on the three-dimensional structure of the protein, if the protein degradation, the antibody reaction will disappear. mAb218 / 17 almost to 1BL. The 2-propanol extract of the 1RS translocation line did not respond but showed a positive color reaction on the other tested wheat lines. Therefore, 1BL. Detection of the 1RS translocation line can be accomplished by a positive reaction to mAb808 / 10 or a negative reaction to mAb218 / 17. In addition, two ELISA runs of a single 2-propanol sample extract were able to identify three populations: 1BL. 1RS translocation system, 1AL. 1RS translocation line and non-translocation of wheat. These ELISA assays have the advantages of simplified procedures, shortened time, high sample screening rates, and the potential to be applied to flour, semolina or semi-grain samples over other Gli-Bl gliadin or Sec-1 rye lecithin immunoassays.