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The aim of this study is to investigate whether three mononucleotide polymorphisms at the locus -1082,-819 and -592 in the promoter region of the IL-10 gene are associated with chronic severe hepatitis.The IL-10-592 and IL-10-1082 polymorphisms were genotyped by polymerase chain reaction- restriction fragment length polymorphism analysis(PCR-RFLP)while polymerase chain reaction-se- quence specific primer(PCR-SSP)assay was used to test the IL-10-819 polymorphism.The polymor- phisms of IL-10-1082,-819 and -592 genes were detected in 98 patients with chronic severe hepatitis (CSH),478 patients with chronic hepatitis B(CHB),223 asymptomatic(chronic)HBV carriers (ASC)and 267 patients with self-restricted HBV.There was significant difference of the polymor- phisms of IL-10-1082,IL-10-819 and IL-10-592 genes between CSH group and other groups.The fre- quency of AA genotype at IL-10 gene promoter -1082 locus in chronic severe hepatitis patients was higher than that in asymptomatic HBV carriers(X~2=13.314,P=0.001),and self-restricted HBV patients(X~2=13.545,P=0.000);the frequency of CC and AC genotype at IL-10 gene promoter -592 locus in chronic severe hepatitis patients was higher than that in chronic hepatitis patients(X~2= 15.970,P=0.000)(X~2=20.414,P=0.000),asymptomatic HBV carriers(X~2=21.283,P= 0.000)(X~2=28.309,P=0.000)and self-restricted HBV patients(X~2=17.047,P=0.000)(X~2 =16.528,P=0.000);the frequency of TC genotype at IL-10 gene promoter-819 locus in chronic severe hepatitis patients was higher than that in chronic hepatitis patients(X~2=58.961,P=0.000), asymptomatic HBV carriers(X~2=53.255,P=0.001)and self-restricted HBV patients(X~2= 39.616,P=0.001).So interleukine-10 gene polymorphism was associated with the chronic severe heoatitis.
The aim of this study is to investigate whether three mononucleotide polymorphisms at the locus -1082, -819 and -592 in the promoter region of the IL-10 gene are associated with chronic severe hepatitis. The IL-10-592 and IL-10 -1082 polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) while polymerase chain reaction-seence specific primer (PCR-SSP) assay was used to test the IL-10-819 polymorphism. - phisms of IL-10-1082, -819 and -592 genes were detected in 98 patients with chronic severe hepatitis (CSH), 478 patients with chronic hepatitis B (CHB), 223 asymptomatic (chronic) HBV carriers (ASC) and 267 patients with self-restricted HBV. There was significant difference of the polymorphisms of IL-10-1082, IL-10-819 and IL-10-592 genes between CSH group and other groups. The fre quency of AA genotype at IL-10 gene promoter -1082 locus in chronic severe hepatitis patients was higher than that in asymptomatic HBV carriers The frequency of CC and AC genotype at IL-10 gene promoter-592 locus in chronic severe hepatitis (X ~ 2 = 13.314, P = 0.001), and self-restricted HBV patients patients were higher than those in chronic hepatitis patients (X ~ 2 = 15.970, P = 0.000) (X ~ 2 = 20.414, P = 0.000) = 28.309, P = 0.000) and the number of self-restricted HBV patients (X ~ 2 = 17.047, P = 0.000) in chronic severe hepatitis patients was higher than those in chronic hepatitis patients (X 2 = 58.961, P = 0.000), asymptomatic HBV carriers (X 2 = 53.255, 39.616, P = 0.001) .So Interleukin-10 gene polymorphism was associated with the chronic severe heoatitis.