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为探讨乙型肝炎病毒前c区基因变异与肝纤维化程度的关系,利用错配聚合酶链反应——限制性片段长度多态性分析检测乙型肝炎病毒前C区1896位点突变,同时联合检测乙型肝炎患者血清透明质酸、层粘连素、Ⅳ型胶原水平,结果183例乙型肝炎患者检出前C区变异85例,急性乙型肝炎无变异,各类慢性乙型肝炎变异率(分别为51.5％、52.8％、57.1％、59.3％、54.8％)尤显著性差异(P>0.05)。而完全变异和变异株优势 变异者慢性乙型肝炎重度和慢性重型肝炎患者明显高于慢性乙型肝炎轻度患者(均P<0.05)。完全变异和变异株优势变异者肝纤维化3项血清学指标明显高于野生株者(均P<0.01)。提示慢性乙型肝炎患者乙型肝炎病毒前C区基因变异普遍存在,且变异程度与病情和肝纤维化程度有关。
To investigate the relationship between the gene mutation of HBV pre-c and the degree of hepatic fibrosis, the 1896-site mutation in pre-C region of hepatitis B virus was detected by mismatch polymerase chain reaction-restriction fragment length polymorphism Serum hyaluronic acid, laminin, and type IV collagen levels were detected in patients with hepatitis B, and the results showed that there were 85 cases of pre-C mutation in 183 cases of hepatitis B patients, no mutation in acute hepatitis B and all kinds of chronic hepatitis B (51.5%, 52.8%, 57.1%, 59.3%, 54.8%, respectively) were significantly different (P> 0.05). However, patients with severe hepatitis B and chronic severe hepatitis were significantly higher than those with mild hepatitis B (all P <0.05). There were three serological markers of liver fibrosis in the mutation of the complete mutation and the mutation of the variant were significantly higher than those in the wild (P <0.01). Prompt hepatitis B virus in patients with pre-C gene mutations prevalence, and the degree of variation and disease severity and the degree of liver fibrosis.