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目的：了解宫颈疾病人乳头状瘤病毒（HPV）感染患者中HPV亚型分布情况及高危型HPV的年龄分布。方法采用专利Invader酶切信号放大法，利用Cervista HPV HR Cervisat核酸杂交基因分型试剂检测宫颈脱落细胞HPV的14个亚型DNA。结果280例宫颈疾病患者，核酸分子杂交检测结果显示，104例感染一种或两种以上的高危型HPV，感染率37.14%，其一种HPV亚型感染率30.00%，混合感染的感染率则为7.14%；宫颈疾病HPV感染者中以高危型HPV 16、43、58、33和6型感染位列前五位；在不同年龄组中以20-50岁组H P V感染率最高。结论本地区宫颈疾病中高危型H P V感染以16、43、58、33和6型单一亚型感染最为常见，且以青壮年为主。“,”Objective To comprehend subtype and age distribution of high risk human papil oma viral infection in cervix disease patients. Method Signal amplification method of patent Invader enzyme digestion was adopted. The 14 HPV subtypes DNA were detected by Cervista- HPV HR Cervisat kit of nucleic acid hybridization. Results In 280 cervix disease patients, the results showed that 104 patients were one and above two HPV subtype infection by hybridization of nucleic acid. The infection rate was 37.14%. Infection rate of single HPV subtype was 30%, but mixed infection rate was 7.14%. HPV 16, 43, 58, 33 and 6 subtypes and mixed HPV were ranked in front fifth of HPV infection patients. Infection rate was the highest in from 20 to 50 years compared with other groups. Conclusion The 16, 43, 58, 33 and 6 subtyps of high risk single HPV and mixed HPV infection were most common. The HPV infection rate was higher in from young and strong population.