论文部分内容阅读
运用ＥＩＴＢ技术分析日本血吸虫（Ｓｊ）尾蚴、肝期童虫及雌雄成虫与旋毛虫（Ｔｓ）肌蚴抗原的交叉性。结果显示：Ｓｊ尾蚴、肝期童虫、成虫各阶段不同程度地被抗－ＴｓＳＡＲＳ，ＴｓＩＲＳ识别，其识别的抗原分子量在１５～１００（尤５０～７０）ｋＤ之间，两种抗血清识别的带型基本相同。所识别的各期Ｓｊ抗原中以尾蚴抗原最多，次为肝期童虫。３０天成虫仅雌虫抗原与上述两种抗血清在９７ｋＤ处显出一条弱带。抗－ＳｊＡＲＳ，ＳｊＩＲＳ识别ＴｓＳＡ中抗原成份分子量基本在３４ｋＤ以上，以３４ｋＤ和３５～３８ｋＤ带最为明显。提示Ｔｓ与Ｓｊ之间存在较多的交叉抗原，主要在幼虫阶段，各阶段之间有较大差异
The EITB technique was used to analyze the cross resistance of Schistosoma japonicum (Sj) cercariae, liver stage sperm and male and female adults with Trichinella spiralis (Ts) sporozoite antigen. The results showed that all the stages of Sj-cercariae, schistosomula and adult worms were identified by anti-TsSARS and TsIRS to varying degrees, and the molecular weight of the identified antigens was between 15-100 (especially 50-70) kD. Belt basically the same. Among the identified Sj antigens, cercaria antigens were the most, followed by liver worms. The 30-day adult female-only female antigen showed a weak band at 97 kD with both antisera. Anti-SjARS and SjIRS recognize that the molecular weight of antigens in TsSA is above 34kD, most notably 34kD and 35 ~ 38kD. Suggesting that there are more cross-antigens between Ts and Sj, mainly in the larval stage, there is a big difference between the various stages