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作者报导的制备特异性高的抗嗜酸性粒细胞血清的方法,对嗜酸性粒细胞机能的研究很有帮助。小白鼠(8周、(?)JCl_1 ICR)经口感染500个犬蛔虫成熟虫卵12日后,末梢血内的嗜酸性粒细胞数显著增多。再往鼠腹腔内注射犬蛔虫成虫提取物,48小时后吸取其腹腔液,每只小白鼠可采取约1×10个嗜酸性粒细胞(腹腔液包含有嗜酸性粒细胞77±3%、巨噬细胞11±2%、淋巴细胞11±4%、嗜中性粒细胞1±1%)。将此腹腔液细胞4×10~3个注射新西兰白兔耳静脉,每间隔一周一次,共注三次。在末次注射后一周放血,分离血清,经灭活置-20℃保存。要获得特异性抗血清时,尚需将此抗血清用小白鼠
The authors report the preparation of specific anti-eosinophil serum method, the study of eosinophil function is very helpful. In mice (8 weeks, (?) JCl? ICR), the number of eosinophils in peripheral blood increased significantly after oral inoculation with 500 adult worms of Ascaris suum. The mice were injected intraperitoneally with the adult worms ascaris extract and the peritoneal fluid was aspirated 48 hours later. Each mouse can take about 1 × 10 eosinophils (peritoneal fluid containing 77 ± 3% eosinophils 11 ± 2% of phagocytes, 11 ± 4% of lymphocytes, and 1 ± 1% of neutrophils). The peritoneal fluid cells 4 × 10 ~ 3 New Zealand white rabbit ear vein injection, once every other week, a total of three injection. One week after the last injection, the blood is excreted and the serum is separated and stored at -20 ° C after inactivation. To obtain specific antiserum, still need to use this antiserum mouse