论文部分内容阅读
为明确栽培环境对铁皮石斛的生长及代谢成分的影响,对铁皮石斛C13品系在岩壁附生、梨树附生和设施盆栽3种栽培环境下进行形态特征、显微结构、多糖和浸出物含量的观察与分析。结果表明,生长环境显著地影响铁皮石斛的生长,在农艺性状上,岩壁附生和梨树附生的铁皮石斛叶片稀少、颜色由绿变紫红、伸展角度减小,茎秆多呈紫色、较粗壮,茎长分别为3.4,4.0 cm,普遍比设施盆栽(7.5 cm)短,且根系特别发达;在显微结构上,野外生长的铁皮石斛与设施栽培比均表现出叶片增厚、上表皮厚度增加、下表皮表皮毛丛生、气孔小而密,根被细胞、外皮层、内皮层的细胞壁均偏厚,且根被、外皮层、内皮层、髓部的细胞小且排列紧密,但栽培环境没有产生特异性的组织结构,主要是结构参数大小和数量的变化,说明铁皮石斛具有很好的环境适应能力;此外,生长环境显著地影响铁皮石斛多糖和醇溶性浸出物等代谢成分的积累,岩壁附生铁皮石斛多糖和醇溶性浸出物含量最高,质量分数分别达37.34%,11.66%,梨树附生次之,质量分数分别为33.90%,9.62%,均高于设施盆栽;通过HPLC分析,岩壁附生铁皮石斛中醇溶性浸出物成分比另外2种栽培模式更加复杂,说岩壁附生栽培环境更有利于铁皮石斛次生代谢产物的合成和积累。
In order to clarify the effect of cultivation environment on the growth and metabolism of Dendrobium candidum, the morphological characteristics, microstructure, polysaccharides and extracts of Dendrobium candidum C13 were studied in three culture environments, Content observation and analysis. The results showed that the growth environment significantly affected the growth of Dendrobium officinale. In agronomic traits, the leaves of epiphyllous camellia and pear tree attached sparsely, the color changed from green to purple, the extension angle decreased, the stems mostly purple, The stems were 3.4 and 4.0 cm in height, respectively, and were generally shorter than that of the potted plants (7.5 cm). The root system was particularly developed. On the microstructure, the growth of wild Dendrobium officinale and its plantation showed thickening of the leaves, Epidermal thickness increased, the epidermal hair under the epidermis, stomata small and dense, root cells, outer layer, the endothelial cell wall are thick, and the root, outer layer, endothelium, medulla cells are small and close arrangement, but The cultivation environment did not produce specific tissue structure, mainly the structural parameters of the size and number of changes, indicating that Dendrobium candidum has a good environmental adaptability; In addition, the growth environment significantly affects the D. officinale polysaccharide and alcohol-soluble extract and other metabolic components The contents of polysaccharides and alcohol-soluble extracts of Dendrobium officinale were the highest, the contents were 37.34% and 11.66%, respectively, and those of pear tree were 33.90% and 9.62%, respectively, The composition of alcohol-soluble extract from Dendrobium officinale was more complicated than the other two cultivation patterns by HPLC analysis. It is concluded that the epiphytic environment of the rock wall is more conducive to the synthesis and accumulation of secondary metabolites of Dendrobium candidum.