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采用聚合酶链反应（ＰＣＲ）从人脑ｃＤＮＡ文库中扩增出６００ｂｐ的髓鞘碱性蛋白（ＭＢＰ）ｃＤＮＡ片段，与载体ｐＧＥＭ－３Ｚｆ（＋）平端连接．重组质粒ＤＮＡ转化宿主菌ＪＭ１０９，在含Ｘ－ｇａｌ和ＩＰＴＧ的平板上直接筛选阳性克隆．限制性内切酶分析和成套引物扩增鉴定证明，该克隆含有７个外显子的２１．５ｋＤ人脑ＭＢＰ全长编码序列．
A 600 bp MBP cDNA fragment was amplified from human brain cDNA library by polymerase chain reaction (PCR) and ligated into vector pGEM-3Zf (+). Recombinant plasmid DNA was transformed into host strain JM109 and the positive clones were screened directly on plates containing X-gal and IPTG. Restriction endonuclease analysis and complete set of primers were used to identify the 21.5kD human MBP full-length coding sequence of seven exons.