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目的:探讨甘肃武都地区宫颈癌患者人乳头瘤病毒(HPV)感染和亚型分布及采用基因芯片检测宫颈癌石蜡标本中人乳头瘤病毒感染的可行性及临床意义。方法:收集2008年5月～2010年5月在甘肃武都地区4所县级医院病理科保存的宫颈癌患者的石蜡组织标本216例,从组织中提取DNA后,应用基因芯片检测21种HPV亚型,即PCR扩增后产物在基因芯片上进行杂交。结果:基因芯片检测结果显示,高危型HPV感染率为87.03%(188/216),低危型HPV感染率为2.78%(6/216),HPV16为主要致病亚型,占50.53%(95/188)、HPV58为第二致病亚型,占21.81%(40/188)、HPV33感染率为11.18%(21/188)、HPV18感染率为9.57%(18/188)。单一感染140例,占72.16%(140/194),混合感染54例,占27.83%(54/194)。其中40例为双重感染,占20.62%(40/194),14例为多重感染,占7.21%(14/194),混合感染多为HPV16合并其它亚型感染。结论:高危型HPV感染是甘肃武都地区宫颈癌发病的重要因素,基因芯片可检测出多种HPV亚型,特异性强、敏感性高,对宫颈癌致病机制的研究及其预防具有重要意义。
Objective: To investigate the distribution and subtype distribution of human papillomavirus (HPV) in patients with cervical cancer in Wudu area of Gansu province and to evaluate the feasibility and clinical significance of human papillomavirus (HPV) infection in cervical cancer samples by gene chip. Methods: Totally 216 paraffin-embedded specimens of cervical cancer patients were collected from four county-level hospitals in Wudu district of Gansu Province from May 2008 to May 2010. DNA was extracted from the tissues and 21 kinds of HPV Subtypes, ie, products after PCR amplification are hybridized on a gene chip. Results: The results of gene chip showed that the infection rate of high-risk HPV was 87.03% (188/216), low-risk HPV infection rate was 2.78% (6/216), HPV16 was the main pathogenic subtype, accounting for 50.53% (95 / 188), HPV58 was the second pathogenic subtype, accounting for 21.81% (40/188), HPV18 infection rate was 11.18% (21/188), HPV18 infection rate was 9.57% (18/188). Single infection in 140 cases, accounting for 72.16% (140/194), mixed infection in 54 cases, accounting for 27.83% (54/194). Among them, 40 cases were double infection, accounting for 20.62% (40/194), 14 cases were multiple infection (7.21%, 14/194). Mixed infection was mostly HPV16 with other subtypes. Conclusion: High-risk HPV infection is an important factor in the pathogenesis of cervical cancer in Wudu Prefecture of Gansu Province. Gene microarray can detect a variety of HPV subtypes with strong specificity and high sensitivity. It is important to study the pathogenesis and prevention of cervical cancer significance.