论文部分内容阅读
目的:探讨早期介入运动训练对脑缺血大鼠脑缺血区血管新生的影响以及对酪氨酸激酶受体Tie-2信号转导通路促进血管新生的调节作用。方法:采用改良线栓法制作大鼠大脑中动脉缺血再灌注模型,将造模成功的大鼠随机分为手术对照组、手术+运动组,同时设立假手术组,并在术后24h后对运动组进行跑台训练。2周后,断头取脑,利用免疫组化方法标记Ⅷ因子检测比较各组大鼠脑缺血区微血管密度;WesternBlot检测各组大鼠脑缺血区血管生成素受体Tie-2、总AKT、磷酸化AKT表达。结果:手术对照组微血管密度较假手术组有所升高,但无统计学意义;与手术对照组相比,手术+运动组微血管密度显著增加并具有统计学意义(P<0.05)。各组大鼠缺血区总AKT、磷酸化AKT、Tie-2表达检测结果:3组总AKT无显著差异;与假手术组相比,手术对照组磷酸化AKT及Tie-2都有升高的趋势,但无统计学意义;手术+运动组较其它两组相比显著升高(P<0.05)。结论:对脑缺血大鼠早期(缺血再灌注损伤24h后)进行2周跑台训练可上调梗死灶边缘区Tie-2受体的表达,促进下游PI3K/AKT通路的相关蛋白磷酸化,启动该通路促进血管新生,增加脑缺血区微血管密度,从而改善大脑血液循环,达到脑保护的作用。
Objective: To investigate the effect of early interventional training on angiogenesis in cerebral ischemic area of rats with cerebral ischemia and the regulation of Tie-2 signal transduction pathway of tyrosine kinase receptor on angiogenesis. Methods: The model of middle cerebral artery occlusion (MCAO) in rats was made by modified suture method. The successful rats were randomly divided into operation control group, operation + exercise group and sham operation group. After 24h Treadmill training exercise group. After 2 weeks, the brain was decapitated, and the Ⅷ-factor was used to detect the microvessel density of cerebral ischemia in each group. Western Blot was used to detect the expression of Tie-2, AKT, phosphorylated AKT expression. Results: The microvessel density of the operation control group was higher than that of the sham operation group, but there was no statistical significance. Compared with the operation control group, the microvessel density of the operation + exercise group increased significantly and had statistical significance (P <0.05). The expression of total AKT, phosphorylated AKT and Tie-2 in the ischemic area of rats in each group showed no significant difference in the total AKT between the three groups. Compared with the sham-operated group, the phosphorylated AKT and Tie-2 in the control group increased But there was no statistical significance. The operation + exercise group was significantly higher than the other two groups (P <0.05). CONCLUSION: Treadmill training in the early stage of ischemia (24h after ischemia-reperfusion injury) can up-regulate the expression of Tie-2 receptor in the marginal zone of infarct and promote the phosphorylation of related protein in the downstream PI3K / AKT pathway. Start the pathway to promote angiogenesis, increased microvessel density of cerebral ischemia, thereby improving cerebral blood circulation, to achieve the role of brain protection.