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目的：实验室选育淡色库蚊胺菊酯抗性品系。方法：ＬＣ５０测定采用浸液法，选育工作参照ＯｍｅｒＳＭｅｔａｌ方法。结果：１９９６年连续用药筛选６代，抗性倍数在第４代曾达到１４倍，尔后种群数量逐渐衰落，至第６代未能继续传代。１９９７年重新选育，共连续用药筛选８代，前３代抗性水平逐步上升，达１３．０７倍；之后逐代下降，第６代降为４．５７倍，第７代有所回升，第８代又有降低。同时进一步研究抗性种群的吸血率、产卵率、各虫期的存活率等种群生存数据发现，与敏感品系相比抗性种群各值均有所下降，种群适合度有一定程度降低，相对适合度为０．２９。结论：本实验中抗胺菊酯品系抗性上升缓慢，可能是胺菊酯的作用机理及其作为击倒剂的作用特点所致。至于抗性种群有无遗传分化，尚需进一步研究。
Objective: To screen the tetramethrin resistant strain in laboratory. Methods: LC50 determination by immersion method, breeding work reference OmerSMetal method. Results: In 1996, six generations of drug were screened continuously, and the resistance multiple was 14 times in the fourth generation. Afterwards, the population gradually declined, and the sixth generation failed to continue the passage. Re-breeding in 1997, a total of eight consecutive drug screening generations, the first three generations of resistance levels gradually increased, up to 13.07 times; after descending generations, the sixth generation dropped to 4.57 times, the seventh generation has picked up, The 8th generation is reduced again. At the same time, we further studied the population survival data of resisting population such as vamping rate, oviposition rate and survival rate at each stage of insects. The results showed that the population of resistant population declined and the fitness of population declined to a certain extent compared with the susceptible lines. The fitness is 0.29. Conclusion: In this experiment, the resistance of anti-tetramethrin strains increased slowly, which may be due to the action mechanism of tetramethrin and its action characteristics as knock-down agent. As for the resistance of the population with or without genetic differentiation, still need further study.