论文部分内容阅读
目的探讨孕期补锌对胎儿生长受限(fetal growth restriction,FGR)仔鼠空间学习记忆能力的影响。方法建造SD孕鼠FGR模型,孕鼠随机分为3组(FGR组10只、模型组10只和正常组11只),模型组喂养添锌饲料,另两组喂养普通饲料。从3组孕鼠中分别随机抽取10只仔鼠作为研究对象(FGR组、模型组均抽取FGR仔鼠)分别于生后1、2、4月进行Morris水迷宫的定位航行实验及空间探索实验,检测FGR子代空间学习记忆能力的改变。结果 1、2、4月龄模型组在水迷宫任务中更多采取有效策略,明显优于FGR组(P<0.05),与对照组间差异无统计学意义(P>0.05)。1、2、4月龄,三组仔鼠的逃避潜伏期随着训练次数的增加而缩短(P<0.05)。1、2、4月龄模型组仔鼠的平均潜伏期较对照组明显增长,差异有统计学意义(P均<0.05)。除4月龄外,模型组仔鼠的平均潜伏期较FGR组明显缩短,站台停留时间较FGR组明显延长(P<0.05),4月龄FGR组、模型组仔鼠站台象限停留时间无统计学意义(P>0.05)。结论孕期补锌能够改善FGR子代大鼠的水迷宫测试成绩,提示孕期补锌对于FGR引起的幼鼠空间学习能力受损有一定的保护作用;出生后行为训练对FGR造成的脑损害可能有一定的纠正作用。
Objective To investigate the effect of zinc supplementation during pregnancy on the spatial learning and memory abilities of fetal growth restriction (FGR) offspring. Methods The FGR model of pregnant SD rats was established. The pregnant rats were randomly divided into three groups (10 in FGR group, 10 in model group and 11 in normal group). The model group was fed with zinc supplemented diet and the other two groups were fed normal diet. Ten pups were randomly selected from the three groups of pregnant rats as experimental subjects (FGR group, model group were taken FGR offspring) were carried out in January, February and April after birth Morris water maze positioning sailing experiment and space exploration experiment , To detect the changes of spatial learning and memory abilities of FGR offspring. Results 1, 2, 4 months old model group more effective strategy in the water maze task, significantly better than the FGR group (P <0.05), and the control group no significant difference (P> 0.05). At 1, 2 and 4 months of age, the escape latency of the offspring in three groups decreased with training times (P <0.05). The average latency of offspring of model rats in 1, 2 and 4 months old group was significantly higher than that of control group (P <0.05). Except for 4-month-old, the average latency of offspring in model group was significantly shorter than that in FGR group, and the stay time in platform was significantly longer than that in FGR group (P <0.05). There was no statistical difference between the 4-month-old FGR group and model group Significance (P> 0.05). Conclusions Zinc supplementation during pregnancy can improve the water maze test scores in FGR offspring rats, suggesting that zinc supplementation during pregnancy may have a protective effect on impaired spatial learning ability induced by FGR. Brain damage caused by postnatal behavioral training may have FGR A certain role in the correction.