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目的 探索荧光原位杂交 (FISH)技术检测稳定性染色体畸变 (易位 )作为辐射生物剂量计的可行性。方法 用 1号、4号全染色体组合探针的FISH技术及常规法检测不同剂量60 Coγ射线诱发的染色体易位率和双着丝粒体率并拟合剂量效应曲线。结果 FISH技术检出的易位率、双着丝粒体率及常规法检出的双着丝粒体率与剂量间均可拟合成线性平方方程Y =c +αD + βD2 。FISH技术和常规法检出的双着丝粒体率差异无显著性 (P >0 0 5 )。对于 1号和 4号染色体 ,辐射诱发的易位率、双着丝粒体率的观察值与基于DNA含量的预期值相比 ,差异皆无显著性。结论荧光原位杂交技术能快速、准确地检测易位且有良好的量效关系 ,因此 ,可望成为估算慢性受照者的累积剂量和早先受照者剂量重建的理想方法
Objective To explore the feasibility of fluorescence in situ hybridization (FISH) to detect stable chromosomal aberrations (translocations) as a radiation biological dosimeter. Methods The chromosomal translocation rate and dicentric mitochondrial rate induced by different doses of 60 Co γ-ray were detected by FISH technique of the first and the fourth whole chromosome combination probes and the routine method was used to fit the dose-response curve. Results The translocation rate, dicentric mitochondrial percentage and dicentromeres detected by FISH were all fitted to the linear square equation Y = c + αD + βD2. There was no significant difference in the rate of dicentric mitochondria detected by FISH and routine methods (P> 0.05). For chromosomes 1 and 4, there was no significant difference between radiation-induced translocation rates and dicentric rates observed compared with those expected based on DNA content. Conclusion Fluorescence in situ hybridization (FISH) can detect translocations quickly and accurately with a good dose-response relationship. Therefore, it is expected to be the ideal method to estimate the cumulative dose of chronic exposure and the dose of previous exposure