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目的:通过db/db小鼠先天性2型糖尿病动物模型,探讨大黄酸对胰岛β细胞第1相胰岛素分泌功能的影响。方法:20只4周龄db/db雄性小鼠,随机分成治疗组和对照组,每组10只。另取10只db/m雄性小鼠作为非糖尿病正常对照。治疗组每日固定时间给予大黄酸120 mg.kg-1灌胃,db/db对照组和db/m对照组均给予相同体积的1%纤维素钠,连续给药8周,测定给药前后小鼠体重和空腹血糖,并于给药结束后分离胰岛,建立离体胰岛灌流系统,开始2.8 mmol.L-1KRB0.5 mL.h-1速度灌流1 h,后换为16.7 mmol.L-1KRB 1 mL.h-1灌流,在-30,-20,-10,0,1/3,2/3,1,4/3,5/3,2,3,4,5,6,7,8,9,10,15,20,30 min时分别收集灌流出的液体,-80℃保存后测定胰岛素。结果:db/db大黄酸治疗组和对照组相比,空腹血糖明显下降。db/db对照组小鼠的离体胰岛第1相胰岛素分泌明显受损,而db/db大黄酸治疗组小鼠离体胰岛在高糖刺激后立即出现明显的第1相胰岛素分泌高峰,较db/db明显好转。结论:早期大黄酸治疗可以明显改善db/db小鼠的葡萄糖耐量,恢复第1相胰岛素分泌功能,保护胰岛功能。
OBJECTIVE: To investigate the effect of rhein on insulin secretion of pancreatic β-cell phase 1 by db / db mouse model of congenital type 2 diabetes. Methods: Twenty male 4-week-old db / db mice were randomly divided into treatment group and control group, with 10 mice in each group. Another 10 db / m male mice as non-diabetic normal control. The treatment group was given rhubarb 120 mg.kg-1 daily fixed time gavage, db / db control group and db / m control group were given the same volume of 1% sodium Cellulose, continuous administration for 8 weeks, before and after administration of the test Mice body weight and fasting blood glucose, and the islets were isolated after the administration, the isolated islet perfusion system was established and the initial perfusion of 2.8 mmol.L-1KRB0.5 mL.h-1 was performed for 1 h and then switched to 16.7 mmol.L- 1KRB 1 mL.h -1 perfusion at -30, -20, -10, 0, 1/3, 2/3, 1, 4/3, 5/3, At 8, 9, 10, 15, 20 and 30 min, the perfusate was collected, and the insulin was measured at -80 ℃. Results: Compared with the control group, the fasting blood glucose of db / db rhein group significantly decreased. db / db control mice were significantly impaired the secretion of phase 1 insulin in isolated islets, and db / db rhein treatment group mice isolated pancreatic islets immediately after high glucose stimulation significant phase 1 insulin secretion peak, compared with db / db significantly improved. Conclusion: Early rhein treatment can significantly improve the glucose tolerance of db / db mice, restore the first phase of insulin secretion and protect the islet function.