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目的:探讨干扰素-γ、干扰素-α能否抑制玻璃体内细胞增生。方法:应用~3H-胸腺嘧啶核苷(~3H-TdR)掺入及液体闪烁测量技术,观察不同浓度的干扰素-γ、干扰素-α(10IU/ml～10~4IU/ml)在3天、5天两个时间点对体外培养的人视网膜色素上皮细胞的影响。结果:在3天时观察,干扰素-γ对人视网膜色素上皮细胞有轻度的抑制作用,最大(10~4IU/ml)抑制率为26%(P<0.05),干扰素-α对视网膜色素上皮细胞有轻度的抑制作用,最大(10~4IU/ml)抑制率为26%(P<0.05)干扰素-α对视网膜色素上皮细胞的抑制作用,较干扰素-γ弱(10~4IU/ml时抑制率为16%)。5天时观察,抑制作用较3天时减弱。结论:干扰素-γ、干扰素-α对人视网膜色素上皮细胞有轻度的抑制作用,其作用维持3天左右。
Objective: To investigate whether interferon-γ and interferon-α can inhibit the proliferation of intravitreal cells. Methods: 3H-thymidine (3H-TdR) incorporation and liquid scintigraphy were used to observe the effects of interferon-γ and interferon-α (10IU / ml ~ 10 ~ 4IU / ml) Day, 5 days two time points on cultured human retinal pigment epithelial cells. Results: At 3 days, interferon-γ showed a mild inhibitory effect on human retinal pigment epithelial cells with a maximum inhibitory rate of 26% (10 ~ 4 IU / ml) (P <0.05). Interferon- The inhibition of epithelial cells was mild (10 ~ 4IU / ml) was 26% (P <0.05). The inhibitory effect of interferon-α on RPE cells was weaker than that of interferon-γ / ml inhibition rate of 16%). Observed 5 days, the inhibitory effect weakened more than 3 days. Conclusion: Interferon-γ and interferon-α have a mild inhibitory effect on human retinal pigment epithelial cells, and their effect is maintained for about 3 days.