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人参皂苷IH901是一种天然二醇组人参皂苷肠道细菌最终代谢产物.目前研究表明,IH901具有抗肝癌活性,然而其作用机理尚不清楚.采用人肝癌细胞株SMMC-7721,HepG2,MHCC97-H来建立体内外的抗肿瘤模型,以探讨人参皂苷IH901的抗肝癌活性及其作用机理.结果发现,人参皂苷IH901能够通过细胞周期中亚二倍体峰的增加、线粒体跨膜电位(ΔΨm)崩溃、DNA梯形条带的形成和提高Caspase-9/Caspase-3的表达量、促进凋亡来抑制肝癌细胞的生长.通过抑制细胞的迁移和降低VEGF/bFGF的表达来抑制肝癌细胞的侵袭转移.裸鼠体内实验均表明了人参皂苷在体内外的抗肝癌活性,并且在分子、细胞、动物水平初步揭示了IH901抗肝癌生长及其侵袭转移的作用机理,为其作为抗肝癌药物的开发提供理论支持.
Ginsenoside IH901 is a natural diol group ginsenoside gut bacteria final metabolite.Studies show that, IH901 has anti-liver cancer activity, but its mechanism of action is not clear.Human hepatoma cell lines SMMC-7721, HepG2, MHCC97- H to establish anti-tumor model in vitro and in vivo to explore the anti-hepatocarcinoma activity of ginsenoside IH901 and its mechanism of action.It was found that ginsenoside IH901 could increase the mitochondrial transmembrane potential (ΔΨm) Collapse, DNA ladder formation and increase the expression of Caspase-9 / Caspase-3, promote apoptosis and inhibit the growth of hepatocellular carcinoma cells by inhibiting the migration of cells and reducing the expression of VEGF / bFGF to inhibit the invasion and metastasis of hepatoma cells In vivo experiments in nude mice have demonstrated the anti-hepatocellular carcinoma activity of ginsenoside in vivo and in vitro, and preliminary revealed the mechanism of action of IH901 on the growth and invasion and metastasis of hepatocellular carcinoma at the molecular, cell and animal levels, and provided the mechanism for its development as an anti-hepatoma drug Theoretical support.