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采用兔离体主动脉条,比较戊脉安(异搏定、Verapamil)对KCl和去甲肾上腺素(NA)引起收缩的影响。在正常Krebs′溶液中,戊脉安对NA所引起的收缩反应的阻遏作用比对KCl为强。对于在无钙Krebs′溶液中放置2小时后的主动脉条,两者的收缩作用都有所减弱,其中NA的收缩减弱程度比KCl更甚;此时如加入戊脉安,可进一步减弱NA所引起的收缩反应,但对KCl无明显影响。如果在无钙Krebs′溶液中仅放置30分钟,KCl引起收缩反应的减弱程度比NA更甚。这可能是由于在无钙营养液中放置了30分钟,仅耗去浅表部位的钙,放置时间的延长(2小时)则深藏部位的钙亦得以减少。根据实验足以阐明戊脉安不仅阻遏兔主动脉平滑肌细胞外钙的内流,而且也可能阻遏细胞内结合钙的释放。
Rabbit isolated aortic strips were used to compare the effects of verapamil on contractions induced by KCl and norepinephrine (NA). In normal Krebs’ solution, verapamil has a more potent contractile response to NA than KCl. The contraction of both aortic strips after 2 hours in calcium-free Krebs’ solution was weakened, in which the contractility of NA was weakened more than that of KCl; at this time, if we added verapamil, the NA could be further weakened Caused by the contraction reaction, but no significant effect on KCl. KCl causes a more pronounced reduction in contractile response than NA if left in a calcium-free Krebs’ solution for only 30 minutes. This may be due to the calcium-free nutrient solution placed in 30 minutes, only to consume superficial calcium, place the extension of time (2 hours) in the deep part of the calcium is also reduced. According to the experiment, it is enough to show that Verapamil not only suppresses the influx of extracellular calcium in rabbit aortic smooth muscle cells, but also may suppress the intracellular calcium release.