A novel organic-inorganic hybrid monolith for trypsin immobilization

• 作 者: WU ShuaiBin MA JunFeng YANG Ka
• 机 构: KeyLaboratoryofSeparationSciencesforAnalyticalChemistry,GraduateUniversityofChineseAcademyofSciences
• 出 处: 中国科学：生命科学英文版
• 发表日期: 2011年1期
• 关键词: 固定化胰蛋白酶 有机无机 杂化 反相液相色谱 牛血清白蛋白 酶反应器 蛋白质组学 串联质谱法 immobilized enzyme reactor organi
• 基金项目: supported by the National Natural Science Foundation of China （Grant Nos. 20935004 and 20775080）, National Basic Research Program of China （Grant No. 2007CB914100）, Knowledge Innovation Program of Chi

In proteomics, attention has focused on various immobilized enzyme reactors (IMERs) for the realization of high throughput digestion. In this report, a novel organic-inorganic hybrid monolith based IMER was prepared in a 100 μm i.d. capillary with 3-glyci