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宿主抵抗小RNA病毒感染依赖于有效的中和抗体应答,CD~+_4 T细胞在诱生中和抗体中起关键作用。然而,迄今对小RNA病毒特异性T细胞的作用以及这些T细胞应答的自然过程及特异性尚知甚少。本文作者首先用脊髓灰质炎病毒1～3型(PV1、PV2、PV3),柯萨基病毒B3、B4(CVB3、CVB4),人类鼻病毒14及脑膜心肌炎病毒(EMCV)等7种不同的小RNA病毒分别与29例正常志愿者外周血单核细胞(PBMCs)进行体外增殖反应。发现大多数志愿者PBMCs均出现特异性增殖,且相互间无显著性差异。继之,作者将PBMCs分别与上述每一小RNA病毒体外培养建立T细胞系,结果所有病毒特异性T细胞系均对上述病毒(除EMCV外)有较高的交叉反应,其中CVB4及PV1～3交叉反应最强;而对热灭活EB病毒、痘苗病
Host resistance to picornavirus infection relies on a potent neutralizing antibody response and CD ~ + _4 T cells play a key role in the induction of neutralizing antibodies. However, the role of picornavirus-specific T cells and the natural processes and specificities of these T cell responses to date are poorly understood. The authors first used seven different small and medium-sized poliovirus types 1 and 3 (PV1, PV2 and PV3), Coxsackie B3, B4 (CVB3 and CVB4), human rhinovirus 14 and meningococcal myocarditis virus The RNA viruses proliferated in vitro in response to peripheral blood mononuclear cells (PBMCs) from 29 normal volunteers, respectively. Found that most of the volunteers PBMCs showed specific proliferation, and no significant difference between each other. Subsequently, the authors established PBMCs with each of the above-mentioned small RNA viruses in vitro to establish a T-cell line. As a result, all the virus-specific T cell lines had high cross-reactivity to the above viruses except EMCV, and among them, CVB4 and PV1- 3 strongest cross-reactivity; and heat-inactivated EB virus, vaccinia disease