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本文进行了不同离子交换剂的选择及静态和动态离子交换纯化蚓激酶的工艺条件研究。结果表明，选用DEAE-纤维素为纯化蚓激酶的离子交换剂，以0．02mol/L，pH7．8磷酸缓冲液+0．5mol/LNaCl作为洗脱剂，蚓激酶纯化效果较好。蚓激酶比活达3．25×104u／mg，比口服用蚓激酶活力提高了3．6倍。
In this paper, the choice of different ion exchangers and static and dynamic ion exchange purification of lumbrokinase process conditions. The results showed that the purification of lumbrokinase was better with DEAE-cellulose as the ion exchanger for the purification of lumbrokinase with 0.02 mol / L pH7.8 phosphate buffer + 0.5 mol / L NaCl as eluent. Lumbrokinase specific activity of 3.25 × 104u / mg, compared with oral administration of lumbrokinase activity 3.6 times.