论文部分内容阅读
目的探讨人乳头瘤病毒(HPV)各亚型感染或多亚型感染与宫颈上皮内瘤变(CIN)的相关性。方法 2007年11月至2008年4月在天津医科大学总医院采用PCR扩增、基因芯片探针杂交分型检测法检测98例CIN患者的HPV亚型。以35岁为界将98例CIN患者分为两组,年龄≤35岁者为A组(43例);年龄>35岁者为B组(55例)。结果 98例CIN中HPV阳性率,CINⅠ为72.41%(21/29),CINⅡ为75%(27/36),CINⅢ为96.97%(32/33)。HPV单重感染率在CINⅠ、CINⅡ和CINⅢ中分别为41.38%(12/29),44.44%(16/36)和57.58%(19/33);HPV双重感染率在CINⅠ、CINⅡ和CINⅢ中分别为27.59%(8/29),19.44%(7/36)和27.27%(9/33);HPV多重感染率在CINⅠ、CINⅡ和CINⅢ中分别为3.45%(1/29),11.11%(4/36)和12.12%(4/33)。CINⅠ中主要为低危型HPV感染,CINⅢ中以HPV16为主导感染亚型。AB两组HPV16感染率分别为20.93%(9/43),40%(22/55),差异具有统计学意义(P<0.05)。结论随CIN病情发展和病变严重,HPV感染率逐渐升高,且双重感染、多重感染均呈升高趋势;HPV感染的亚型不同。CINⅢ中的主要HPV亚型为HPV16、58;HPV的双重或多重感染可能促进了CIN的进程。预防或控制HPV16、58感染,将大大减少CINⅢ及宫颈浸润癌的发生。
Objective To investigate the relationship between subtypes of human papillomavirus (HPV) infection and multiple subtype infection and cervical intraepithelial neoplasia (CIN). Methods From November 2007 to April 2008, HPV subtypes of 98 CIN patients were detected by PCR amplification and gene chip probe hybridization typing in Tianjin Medical University General Hospital. A total of 98 patients with CIN were divided into two groups according to the age of 35 years old. Group A (43 cases) ≤35 years old and Group B (55 cases)> 35 years old. Results The positive rates of HPV in 98 cases of CIN were 72.41% (21/29) for CINⅠ, 75% (27/36) for CINⅡ and 96.97% (32/33) for CINⅢ. The infection rate of HPV was 41.38% (12/29), 44.44% (16/36) and 57.58% (19/33) in CINⅠ, CINⅡ and CINⅢ respectively. The infection rates of HPV in CINⅠ, CINⅡ and CINⅢ were respectively Were 27.59% (8/29), 19.44% (7/36) and 27.27% (9/33), respectively. The multiple infection rates of HPV in CINⅠ, CINⅡ and CINⅢ were 3.45% (1/29) and 11.11% / 36) and 12.12% (4/33). CIN Ⅰ mainly low-risk HPV infection, CIN Ⅲ in HPV16-predominant subtype of infection. The infection rates of HPV16 in two groups were 20.93% (9/43) and 40% (22/55), respectively, with significant difference (P <0.05). Conclusions With the development of CIN and serious disease, the infection rate of HPV is gradually increased, and the double infection and multiple infection all show an increasing trend. The subtypes of HPV infection are different. The major HPV subtype in CINIII is HPV16,58; dual or multiple HPV infection may promote the progression of CIN. Prevention or control of HPV16,58 infection, will greatly reduce the incidence of CIN Ⅲ and invasive cervical cancer.