In vitro activities of acetonic extracts from leaves of three forage legumes (Calliandra calotyrsus,

• 作 者: Wabo Pon J Kenne Tameli Florence Mpoame Mbida Pamo Tedonkeng E Bilong Bilong CF
• 机 构: Department of Animal Biology, Faculty of Science, University of Dschang, P 0 Box 067 Dschang, Camero
• 出 处: 亚太热带医药杂志（英文版）
• 发表日期: 2011年2期
• 关键词: Extract Calliandra calotyrsus Gliricidia sepium Leucaena diversifolia Mebendazol

Objective:To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume,Calliandra calotyrsus (C. calotyrsus),Gliricidia sepium (G. sepium) andLeucaena diversifolia (L. diversifolia) in vitro onHaemonchus contortus (H. contortus).Methods: Eggs were exposed for 24 hours to five different concentrations (0.075, 0.15, 0.3, 0.6 and1.25mg/mL) of acetonic extracts at room temperature (24 ℃). Distilled water and0.4% Tween were used in the bioassay as negative controls.Results: The later did not affect embryonation and egg hatching ofH. contortus. Conversively, significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL). The acetonic extract ofG. sepium was found to be more active (2.9% and0.0% for embryonation and egg hatching, respectively) than the other substances16.5% and33.5%, respectively forC. calothyrsus,33.7% and33.3%, respectively forL. diversifolia.Conclusions:These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out.