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RNAscope是以新型探针设计为优势的新一代原位杂交技术，现正逐步应用于多个研究领域，研究范围亦在不断拓展之中。我国肝病人口基数大，基于组织学生物信息检测及研究评价的需求性大，该技术有独特原位水平的特异性与敏感性，弥补了免疫组织化学与传统原位杂交的技术缺陷。实现多重性及单分子水平检测，可在肝病领域多个方面进行核酸水平的定性、定量分析并与组织原位结合实现可视化评价以凸显“金标准”的价值。现就近年来RNAscope技术在肝脏组织学方面的应用进行综述。“,”RNAscope is a new generation of in situ hybridization technology and with the advantage of new probe design, it is now being gradually applied to a wide range of research fields, and its research scope is constantly expanding. Our country has large number of liver disease patients, so there is a great demand for histological testing and research evaluation based upon biological information. This technology has unique specificity and sensitivity in situ level, which makes up for the technical defects of immunohistochemistry and traditional in situ hybridization. Realizing the multiplicity and monomolecular level detection, the qualitative and quantitative analysis of nucleic acid level can be carried out in many aspects of the field of liver disease, and the visual evaluation can be achieved by combining it with tissue in situ to highlight the value of “gold standard”. This article summarizes the recent year’s application of RNAscope technology carried out in liver histology field.