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二甲基甲酰胺(DMF)是一种广泛使用的有机溶剂,文献表明它是一种亲肝性毒物,其毒作用机理尚未阐明。近来研究发现,细胞内钙稳态失调与其造成的细胞损伤密切相关,细胞内钙离子浓度升高是许多外源性化合物导致细胞死亡的最终途径。本文采用胶原酶灌流法分离制备大鼠肝细胞悬
Dimethylformamide (DMF) is a widely used organic solvent, and the literature shows that it is a hepatotropic poison whose mechanism of action has not yet been elucidated. Recent studies have found that intracellular calcium homeostasis is closely related to the cellular damage caused by it. The increase of intracellular calcium concentration is the ultimate pathway for many exogenous compounds to cause cell death. In this paper, collagenase perfusion method for the preparation of rat hepatocyte suspension