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目的为探讨结肠恶性淋巴瘤的发病机制和实验治疗提供理想的动物模型。方法将结肠恶性淋巴瘤术中原发灶和肝转移灶新鲜瘤组织块植入裸小鼠结肠黏膜层内,观察原位移植的成瘤率,移植瘤的侵袭和转移率。进行形态学(光镜、电镜、免疫组织化学)、染色体核型和流式细胞分析。结果人结肠淋巴瘤原发灶和肝转移灶新鲜瘤组织均获得移植成功。依据WHO新的分类标准,建成1株人结肠原发性(原发灶)非霍奇金B细胞性恶性淋巴瘤裸鼠原位移植高转移模型(HCBL-0303)和1株人结肠原发性(肝转移灶)非霍奇金B细胞性恶性淋巴瘤裸鼠原位移植肝转移模型(HCBL-0304)。移植瘤组织病理学为(非霍奇金B细胞性)高度恶性淋巴瘤；免疫组化显示CD19、CD20、CD22阳性。CD3、CD7阴性。染色体数目55～59条；流式细胞DI值1．59～1．71,均为异倍体。HCBL-0303肝转移率为63．7%,淋巴结转移率为56．4%；HCBL-0304肝转移率和淋巴结转移率为100%。移植瘤在裸鼠结肠内自主侵袭性生长,发生血液转移、淋巴转移和腹腔内种植性转移。结论HCBL-0303和HCBL-0304是首次成功建立的人结肠恶性淋巴瘤裸鼠原位移植自发性肝转移模型,可用于结肠恶性淋巴瘤的发病机制、侵袭、转移及实验治疗的研究。
Objective To investigate the pathogenesis and experimental treatment of colorectal malignant lymphoma to provide an ideal animal model. Methods The primary tumor of colorectal malignant lymphoma and the new tumor tissue of liver metastasis were implanted into the colonic mucosa of nude mice. The rate of tumorigenicity in situ transplantation and the invasion and metastasis of the transplanted tumor were observed. Morphology (light microscopy, electron microscopy, immunohistochemistry), chromosomal karyotype and flow cytometry were performed. Results The primary tumor of human lymphoma and the new tumor of liver metastasis were all successfully transplanted. According to the new classification standard of WHO, one human colorectal primary (primary tumor) non-Hodgkin’s B-cell malignant lymphoma in nude mice orthotopic transplantation high metastasis model (HCBL-0303) and one human primary colon (Liver metastases) Non-Hodgkin’s B-cell malignant lymphoma orthotopic liver transplantation model in nude mice (HCBL-0304). Histopathology of the xenografts was (non-Hodgkin’s B-cell) highly malignant lymphoma; immunohistochemistry showed positive for CD19, CD20 and CD22. CD3, CD7 negative. Chromosome number 55 to 59; flow cytometry DI values 1.59 ~ 1.71, are aneuploid. HCBL-0303 liver metastasis rate was 63.7%, lymph node metastasis rate was 56.4%; HCBL-0304 hepatic metastasis rate and lymph node metastasis rate was 100%. Transplanted tumor in the colon of nude mice autonomously aggressive growth, blood metastasis, lymphatic metastasis and intra-abdominal implant metastasis. Conclusions HCBL-0303 and HCBL-0304 are the first successful models of orthotopic liver transplantation in human colonic lymphoma in nude mice, which can be used to study the pathogenesis, invasion, metastasis and experimental treatment of colorectal malignant lymphoma.