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目的观察血清和血浆标本不同处理方法和时间对外周血可溶型CD100(sCD100)水平的影响。方法用未加促凝剂血清采血管、含硅化涂层促凝管以及肝素抗凝管、乙二胺四乙酸(EDTA)抗凝管和枸橼酸钠抗凝管,分别在凝血1、4、8 h,分别收集2种血清收集管内血清标本。采用ELISA检测标本中sCD100水平,比较不同标本和时间处理对sCD100检测水平的影响。结果不同抗凝剂处理后的血浆标本中,sCD100检测结果无明显差异。血清标本sCD100水平明显高于血浆标本。使用促凝剂血清sCD100水平高于无促凝剂血清sCD100水平。在室温条件下,血清标本随着凝血时间的延长,sCD100水平逐渐升高。结论血清中sCD100水平显著高于血浆,处理时间延长可引起血清sCD100水平进一步增高。在定量检测白细胞和血小板膜分子的可溶型标志物时,应注意标本收集过程中的标准化。
Objective To observe the effects of different treatment methods and time on the level of soluble CD100 (sCD100) in peripheral blood of serum and plasma samples. Methods Blood samples were collected without coagulant serum, coagulant containing silicidation and heparin anticoagulant, ethylenediaminetetraacetic acid (EDTA) anticoagulant and sodium citrate anticoagulant respectively. , 8 h, respectively, collected two serum collection tube serum samples. The level of sCD100 was detected by ELISA, and the effect of different samples and time on the level of sCD100 was compared. Results Different anticoagulant plasma samples, sCD100 test results no significant difference. Serum sCD100 levels were significantly higher than plasma samples. The level of sCD100 using coagulant serum was higher than that of sCD100 without coagulant serum. At room temperature, serum samples with clotting time, sCD100 levels gradually increased. Conclusion Serum sCD100 levels were significantly higher than plasma, prolonged treatment can cause serum sCD100 levels further increased. When quantitatively detecting soluble markers of leukocytes and platelet membrane molecules, attention should be paid to the standardization during specimen collection.