论文部分内容阅读
目的:研究P-糖蛋白抑制剂对蝙蝠葛碱肠吸收的影响。方法:采用在体单向肠灌流法进行小肠吸收实验,利用HPLC法测定灌流液中蝙蝠葛碱的浓度,考察不同浓度P-糖蛋白抑制剂环孢素A、醋酸地塞米松和维拉帕米对蝙蝠葛碱肠吸收的影响。结果:与对照组相比,高浓度和中浓度环孢素A对蝙蝠葛碱的Ka、Papp、P%、吸收量和累积吸收量均有显著性影响(P<0.05),而低浓度时上述参数差异无显著性(P>0.05);与对照组相比,高、中、低3个浓度醋酸地塞米松对蝙蝠葛碱的Ka、Papp、P%、吸收量和累积吸收量均有显著性影响(P<0.05);与对照组相比,高浓度维拉帕米对Dau的Ka、Papp、P%、吸收量和累积吸收量均有显著性影响(P<0.05),而中浓度和低浓度时上述参数无显著性差异(P>0.05)。结论:P-糖蛋白抑制剂环孢素A、醋酸地塞米松和维拉帕米对蝙蝠葛碱均有促吸收作用,其作用大小顺序为醋酸地塞米松>环孢素A>维拉帕米;P-糖蛋白对Dau的肠吸收有外排作用,蝙蝠葛碱为P-糖蛋白底物。
Objective: To study the effects of P-glycoprotein inhibitors on the absorption of daulium saurinosae. Methods: The intestine absorption experiment was carried out in vivo. The concentration of dauricine in the perfusate was determined by HPLC. The effects of cyclosporin A, dexamethasone acetate and verapamil Effect of Rice on the Absorption of Alkali in Dwarf. Results: Ka, Papp, P%, uptake and cumulative uptake of daulbin by high and medium concentrations of cyclophosphamide A were significantly higher than those of the control group (P <0.05) There was no significant difference between the above parameters (P> 0.05). Compared with the control group, Ka, Papp, P%, absorption and cumulative absorption of three concentrations of dexamethasone acetate at high, (P <0.05). Compared with the control group, the high concentration of verapamil had a significant effect on Ka, Papp, P%, absorption and cumulative absorption of Dau (P <0.05) There was no significant difference in the above parameters between the concentration and the low concentration (P> 0.05). CONCLUSION: P-glycoprotein inhibitors cyclosporin A, dexamethasone acetate and verapamil have an anti-absorption effect on dauricine. The order of action is dexamethasone acetate> cyclosporin A> verapamil Rice; P-glycoprotein efflux of intestinal absorption of Dau, dauricine P-glycoprotein substrate.