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目的:了解卵巢切除(OVX)大鼠胫骨对氟化钠和尼尔雌醇联合应用的治疗反应。方法:将24只雌性大鼠随机分成3组:OVX组、对照组和EF组,每组8只。对OVX组和EF组大鼠行OVX手术,建立骨质疏松动物模型。EF组于OVX术后1个月予氟化钠和尼尔雌醇联合治疗,给药3个月后处死。用骨形态计量学方法检测OVX大鼠左后肢胫骨对氟化钠的治疗反应。结果OVX组大鼠骨小梁体积、平均骨小梁密度较对照组显著减少(P<0.05);四环素标记表面、类骨质表面和组织水平的骨形成速率均较对照组显著增加(P<0.05);矿化沉积速率、类骨质成熟时间和矿化延迟时间,较对照组缩短(P<0.05)。EF组治疗12周后发现,骨小梁体积和平均骨小梁宽度高于OVX组(P<0.05),其中TBW显著高于对照组(P<0.05);骨骺部位MTPD和MTPS与对照组比较无显著差异(P>0.05)。EF治疗组骨小梁的四环素标记表面、类骨质表面、组织水平骨形成速率和平均类骨质宽度低于OVX组(P<0.05),矿化沉积速率和矿化延迟时间低于对照组(P<0.05)。结论:尼尔雌醇和氟化钠联合治疗可显著增加平均骨小梁宽度和骨小梁骨量,并改善小梁骨空间结构。骨骺部位的改善作用较为明显。
OBJECTIVE: To understand the therapeutic response of the tibia of ovariectomized (OVX) rats to the combined use of sodium fluoride and nylestriol. Methods: Twenty-four female rats were randomly divided into 3 groups: OVX group, control group and EF group, 8 rats in each group. OVX group and EF group underwent OVX surgery, animal model of osteoporosis. The EF group was treated with sodium fluoride and nylestriol one month after OVX. After 3 months, the mice were sacrificed. Bone histomorphometry was used to examine the response of tibia in left hindlimb to sodium fluoride in OVX rats. Results Compared with the control group, the trabecular bone volume and the average trabecular bone density were significantly decreased in the OVX group (P <0.05). The bone formation rates at the tetracycline labeled surface, osteoid surface and tissue level were significantly higher than those in the control group (P < 0.05). The mineralization deposition rate, osteoid maturation time and mineralization delay time were shorter than those in the control group (P <0.05). After 12 weeks of treatment, the trabecular bone volume and mean trabecular width were significantly higher in EF group than those in OVX group (P <0.05), and the TBW in EF group was significantly higher than that in control group (P <0.05). MTPD and MTPS in epiphyseal part were significantly higher than those in control group No significant difference (P> 0.05). In the trabecular group, the tetracycline-labeled surface, osteoid surface, tissue-level bone formation rate and mean bone width were lower in the trabecular group than those in the OVX group (P <0.05), and the mineralization deposition rate and mineralization delay time were lower than those in the control group (P <0.05). Conclusion: The combination of nylestriol and sodium fluoride can significantly increase the average trabecular bone width and trabecular bone mass, and improve the trabecular bone spatial structure. Epiphyseal site to improve the role of more obvious.