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目的:探讨硫酸镁对继发性脊髓损伤的保护作用及其作用机制。方法:选健康新西兰大白兔36只,随机分为3组:A组为正常组,仅行L1￣L3椎板减压;B组和C组分别为对照组和治疗组,行L1￣L3椎板减压后采用Allen′s重物打击法致伤脊髓,伤后30min时B组经腹腔注射蒸馏水600mg/kg,C组经腹腔注射硫酸镁600mg/kg。48h后切取伤段脊髓组织分别测定水、钙、镁含量,观察局部组织病理学改变、超微结构变化及单位面积凋亡细胞数。结果:与A组比较,B、C组伤段脊髓组织水、钙含量增多,镁含量减少,组织病理学改变及超微结构破坏严重,细胞凋亡数上升,且C组较对B组轻。结论:早期应用硫酸镁治疗可减轻脊髓损伤后的继发性损伤。
Objective: To investigate the protective effect of magnesium sulfate on secondary spinal cord injury and its mechanism. Methods: Thirty-six healthy New Zealand white rabbits were randomly divided into 3 groups: group A was normal group, only L1 ~ L3 vertebral plate decompression; group B and group C were control group and treatment group respectively, L1 ~ L3 vertebra After decompression, the injured spinal cord was injuried with Allen’s heavy blow method. Group B received intraperitoneal injection of distilled water 600 mg / kg at 30 min after injury and Group C received intraperitoneal injection of 600 mg / kg magnesium sulfate. After 48h, the injured spinal cord tissue was cut to determine the contents of water, calcium and magnesium respectively. Local histopathological changes, ultrastructural changes and apoptotic cells per unit area were observed. Results: Compared with group A, the content of water and calcium in spinal cord of groups B and C increased, the content of magnesium decreased, the histopathological changes and the ultrastructure were damaged severely, the number of apoptosis increased, and in group C than in group B . Conclusion: The early application of magnesium sulfate can reduce the secondary injury after spinal cord injury.