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目的:通过鼠肿瘤动物模型确定ALA口服激光光动力疗法促使肿细胞死亡及凋亡的疗效及光敏药物最佳用药剂量。方法:以不同剂量的ALA口服(20μg/ml、40μg/ml、60μg/ml、120μg/ml、240μg/ml)、以630nm半导体激光200mW/cm2照射,每光斑照射20分钟剂量,照光前及照光后不同时间以肉眼、肿瘤大小测定、病理、电镜、流式细胞仪观察疗效差别并与未作光动力学治疗的空白对照组模型作比较,寻找最佳的光敏药物配合的剂量。结果:随ALA口服剂量增加PDT疗效增加,口服剂量达60mg/kg疗效明显增加能抑止肿瘤生长,肿瘤变暗,质变硬,结痂,60mg/kg,120mg/kg,240mg/kg疗效相似,并不能达到完全的杀除肿瘤,该法作为肿瘤治疗若与HPD-PDT联合使用,可提高疗效,减少HPD剂量,减少避光时间,ALA-PDT可作肿瘤诊断,可避免了HPD光敏诊断的皮肤光毒反应。结论:光动力学治疗脑瘤口服剂量达60mg/kg疗效明显增加,能抑止肿瘤生长,剂量再增加疗效相似,并不能达到完全的杀灭肿瘤。
OBJECTIVE: To determine the efficacy of ALA oral laser photodynamic therapy in promoting the death and apoptosis of swollen cells and the optimal dosage of photosensitizer by using mouse tumor animal models. Methods: The dose of ALA was given orally (20μg / ml, 40μg / ml, 60μg / ml, 120μg / ml, 240μg / ml) with 630nm semiconductor laser at 200mW / cm2. At different times, the difference of efficacy was observed by naked eye, tumor size, pathology, electron microscopy and flow cytometry, and compared with the blank control group without photodynamic therapy to find out the optimal dose of photosensitizer. Results: With the increase of oral dosage of ALA, the efficacy of PDT increased. The oral dosage of 60mg / kg could significantly inhibit the growth of tumor, diminish the tumor, harden the texture and scab. The curative effects of 60mg / kg, 120mg / kg and 240mg / kg were similar Can not achieve completely kill the tumor, the law as a tumor treatment if combined with HPD-PDT, can improve the efficacy, reduce the HPD dose, reduce the dark time, ALA-PDT can be used for tumor diagnosis, to avoid photosensitive skin of HPD diagnosis Photo poisoning reaction. Conclusion: Photodynamic therapy of brain tumor oral dose of 60mg / kg significantly increased, can inhibit tumor growth, dose and then increase the similar effect, and can not achieve complete killing of the tumor.