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目的研究purmorphamine在动态张应力促人牙周膜干细胞(human periodontal ligament stem cells,PDLSCs)向成骨细胞分化过程中的作用。方法分离培养鉴定PDLSCs,在矿化诱导环境中加入purmorphamine,采用Flexcell FX-4000T应力加载系统对细胞加力24 h,以Real-time PCR检测成骨相关指标Runx2、alkaline phosphatase(ALP)以及Hedgehog(Hh)通路的标志物GLI1、Pathed1(PTCH1)、Smoothend(SMO)。结果动态张应力作用24 h后,成骨相关指标Runx2、ALP,Hh通路的标志物GLI1、PTCH1、SMO的表达水平明显升高(P<0.05);加入purmorphamine后,成骨相关指标Runx2、ALP,Hh通路的标志物GLI1、PTCH1、SMO的表达水平较加力组均有增强,差异有统计学意义(P<0.05)。结论 purmor-phamine可能通过激活Hh通路,进而增强人PDLSCs应力条件下向成骨细胞分化。
Objective To investigate the role of purmorphamine in osteoblast differentiation induced by dynamic tensile stress on human periodontal ligament stem cells (PDLSCs). Methods PDLSCs were isolated and identified, and purmorphamine was added into the mineralizing environment. The cells were loaded with Flexcell FX-4000T stress system for 24 hours. Real-time PCR was used to detect osteoblast-related indicators Runx2, alkaline phosphatase (ALP) and Hedgehog Hh) pathway markers GLI1, Pathed1 (PTCH1), Smoothend (SMO). Results After 24 h of dynamic tensile stress, the expressions of GLI1, PTCH1 and SMO in Runx2, ALP and Hh pathways were significantly increased (P <0.05). After addition of purmorphamine, the expressions of Runx2, ALP , And the expression of GLI1, PTCH1 and SMO in Hh pathway were all significantly higher than that in the stress group (P <0.05). Conclusion Purmor-phamine may promote the differentiation of osteoblasts by activating the Hh pathway and further enhancing the stress of human PDLSCs.