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将具有光电活性的硫堇电聚合在光透电极的表面,制备了聚硫堇膜光电极,利用该电极同时具有光敏和电子受体的性能,使其与葡萄糖氧化酶对葡萄糖催化反应产生的电子供体(过氧化氢)发生光致电化学反应,通过检测由此产生的光电流实现对蛋白质催化活性的检测.本文探讨了聚硫堇/过氧化氢光致电化学敏感界面的响应机理,讨论了偏压、电解质溶液pH和底物浓度对光致电化学反应和测定葡萄糖氧化酶催化活性的影响.通过测定商品葡萄糖氧化酶的催化活性,验证了方法的可行性.应用该敏感界面检测蛋白质的催化活性不需要过氧化物酶,比光度法经济、简便和快速.
The photoactive thionine was electropolymerized on the surface of the light transmitting electrode to prepare polythionine photoelectrode. The photoelectrode has the properties of photoactive and electron acceptor, which can react with glucose oxidase to catalyze the reaction of glucose Photoelectrochemical reaction of electron donor (hydrogen peroxide), detection of the photocatalytic activity of the protein by detecting the photocurrent generated.This paper discusses the response mechanism of the polythionine / hydrogen peroxide sensitive interface of the photoelectrochemistry to discuss The effects of bias voltage, electrolyte solution pH and substrate concentration on the photoelectrochemical reaction and the determination of glucose oxidase catalytic activity were investigated.The feasibility of the method was validated by measuring the catalytic activity of commercial glucose oxidase Catalytic activity does not require peroxidase, than the photometric method is economical, simple and fast.