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在离体条件下，采用噻唑蓝（MTT）染色法，观察干扰素（IFN）与丝裂霉素C（MMC）、阿霉素（ADM）、噻替哌（TSPA）和羟基喜树碱（HCPT）等化疗药物联合应用对人类膀胱癌细胞株BIU－87的抑制作用。结果在连续联合用药组，浓度为25000u／ml的IFN与上述药物50％抑制率（ID50－1）剂量联合使用的抑制率分别为57．4％、58．6％、55．3％和64．3％；在序贯联合用药组，将MMC、ADM的ID50－2剂量作用于细胞24h后，再使用浓度为25000u／ml的IFN，24h后两者联合使用的抑制率分别为59．4％和54．3％。认为IFN与MMC等化疗药物联合应用能够取得较好的协同作用，为临床治疗浅表性膀胱肿瘤提供了新的思路。
In vitro, the effects of interferon (IFN) and mitomycin C (MMC), doxorubicin (ADM), thiotepa (TSPA) and hydroxycamptothecin HCPT) and other chemotherapy drugs on human bladder cancer cell line BIU-87 inhibition. Results In the continuous combination group, the inhibitory rates of combined use of IFN at the concentration of 25000u / ml and the 50% inhibitory rate (ID50-1) of the above drugs were 57.4%, 58.6%, 55.3% and 64 .3%; In the sequential combination group, the MMC, ADM ID50-2 dose after 24h, then the concentration of 25000u / ml of IFN, 24h combined inhibition rate of the two were 59.4 % And 54.3%. That IFN and MMC and other chemotherapy drugs can achieve a better synergistic effect, for the clinical treatment of superficial bladder tumors provide a new way of thinking.