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本文用HPLC法测定清开灵注射液中的有效成份黄芩甙和绿原酸的含量。本方法简便,快速,重现性好,为清开灵注射液质量控制提供了可靠的检测手段。检测波长340nm,ODS(C—18)、3.9mm×30cm色谱柱,流动相为甲醇-水-四氢呋喃(25:57:18)。实验部分一、仪器与试剂 1、仪器:美国Waters高效液相色谱仪810系统;490E紫外检测器;745数据处理机;510型泵,U6K进样器。 2、试剂:甲醇,四氢呋喃,磷酸均为优级纯超纯水。 3、标准及样品:黄芩甙,绿原酸,异补骨脂素(北京市药品检定所提供)。清开灵注射液(北京中医学院实验药厂提供)。二、色谱条件不锈钢色谱柱:YWG ODS(c-18)5μm,3.9mm×30cm
In this paper, HPLC determination of Qingkailing injection of active ingredient baicalin and chlorogenic acid content. The method is simple, rapid, reproducible, and provides a reliable means of detection for the quality control of Qingkailing Injection. Detection wavelength 340nm, ODS (C-18), 3.9mm × 30cm column, the mobile phase is methanol - water - tetrahydrofuran (25:57:18). Experimental part A, instruments and reagents 1, instruments: the United States Waters high performance liquid chromatography 810 system; 490E UV detector; 745 data processor; 510 pump, U6K injector. 2, Reagents: methanol, tetrahydrofuran, phosphoric acid are excellent pure pure water. 3, standards and samples: baicalin, chlorogenic acid, isopsoralen (Beijing Institute of Drug Control). Qing Kai Ling Injection (Beijing Institute of Traditional Chinese Medicine Laboratory). Second, the chromatographic conditions Stainless steel column: YWG ODS (c-18) 5μm, 3.9mm × 30cm