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目的探讨端粒长度的变化与胃癌形成的关系。方法采集相对正常胃黏膜10例、癌前病变15例、胃癌20例、淋巴结转移癌组织5例;Southern blot分析端粒长度的变化。实验数据采用方差分析,P<0.05有统计学意义。结果正常胃黏膜、癌前病变、胃癌及淋巴结转移癌组织平均端粒长度分别为(9.20±1.09)、(8.70±0.81)、(7.66±1.77)及(7.30±0.42)kb。方差分析癌前病变组、胃癌组和转移癌组的端粒长度比正常组短;转移癌组、胃癌组的端粒长度比癌前病变组短(P<0.01)。胃癌中端粒长度与患者的年龄、性别、肿瘤大小、肿瘤的分化程度和临床分期无关(P>0.05)。结论正常胃黏膜、癌前病变、胃癌与转移癌组织的平均端粒长度逐渐缩短。端粒长度的改变可能参与胃癌形成过程,端粒缩短可能是胃癌发生的早期事件。
Objective To investigate the relationship between telomere length change and gastric carcinogenesis. Methods 10 cases of normal gastric mucosa, 15 cases of precancerous lesions, 20 cases of gastric cancer and 5 cases of lymph node metastasis were collected. The telomere length was analyzed by Southern blot. The experimental data using analysis of variance, P <0.05 was statistically significant. Results The average telomere length of normal gastric mucosa, precancerous lesions, gastric cancer and lymph node metastasis was (9.20 ± 1.09), (8.70 ± 0.81), (7.66 ± 1.77) and (7.30 ± 0.42) kb, respectively. Analysis of variance showed that telomere length in precancerous lesions group, gastric cancer group and metastatic cancer group was shorter than that in normal control group. Telomere length in metastatic cancer group and gastric cancer group was shorter than that in precancerous lesions group (P <0.01). The telomere length in gastric cancer was not related to the patient’s age, sex, tumor size, tumor differentiation and clinical stage (P> 0.05). Conclusion The average telomere length of normal gastric mucosa, precancerous lesions, gastric cancer and metastatic cancer tissues is gradually shortened. Changes in telomere length may be involved in the process of gastric carcinogenesis. Telomere shortening may be an early event in gastric carcinogenesis.