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目的:探索唐古特大黄蒽醌代谢物可能的前体,初步判断其合成途径。方法:选用唐古特大黄种子萌发的无菌苗为材料,诱导愈伤组织,研究添加不同前体、不同饲喂时间对唐古特大黄愈伤组织和成体大黄蒽醌产量的影响。结果:乙酸对蒽醌产量的促进作用最大,在愈伤组织中达到了43.9%的增幅,在成体大黄中达到了45.8%;丙二酸其次,在愈伤组织中的增幅达到了15.8%,但在成体大黄中却只有3.6%的增长。苯甲酸和对苯醌对蒽醌产量无明显影响,莽草酸和α-酮戊二酸对蒽醌产量反而有一定的抑制作用。36 h是比较合适的饲喂时间,可将前体的饲喂效果充分发挥出来。结论:乙酸是唐古特大黄蒽醌代谢物合成的前体,它能大幅度的提高愈伤组织和成体大黄中蒽醌的产量,并由此推断出唐古特大黄蒽醌代谢物的合成途径是多酮合成途径。
OBJECTIVE: To explore the possible precursors of anthraquinone metabolites from rhubarb of Tanggut, and to determine its synthesis pathway. Methods: The germ-free germ of Tanggut rhubarb seed was used as material to induce the callus, and the effects of adding different precursors and feeding time on the production of the rhubarb callus and the adult anthraquinones were studied. Results: Acetic acid had the greatest effect on anthraquinone yield, reached 43.9% increase in callus and reached 45.8% in adult rhubarb; followed by malonic acid in callus increased by 15.8% However, there is only 3.6% increase in adult rhubarb. Benzoic acid and p-benzoquinone had no significant effect on anthraquinone production, while shikimic acid and α-ketoglutarate had some inhibitory effect on anthraquinone production. 36 h is a more suitable feeding time, which can give full play to the feeding effect of the precursor. CONCLUSION: Acetic acid is the precursor of the anthraquinone metabolites of rhubarb of Rheum tanguticum, which can greatly increase the production of anthraquinones in callus and adult rhubarb. It is concluded that the synthetic pathway of anthraquinone metabolites of rhubarb .