论文部分内容阅读
目的 探讨乙型肝炎病毒 (HBV)慢性感染者血清白细胞介素 -12(IL-12)、干扰素 -γ(IFN -γ)和白细胞介素 -10(IL-10)检测的临床意义。方法 分离97例HBV慢性感染者血清 ,其中41例血清丙氨酸转移酶 (ALT)水平正常 ,56例血清ALT水平异常。采用双抗体夹心ELISA方法检测血清IL-12,IFN -γ和IL-10水平。结果 HBV慢性感染者血清ALT正常组和ALT异常组的血清IFN-γ水平分别为 (16.62±15.15)和 (24.91±48.68)ng/L,均比正常对照组水平 (6.25±2.22)ng/L显著增高 (均P<0.01) ;ALT异常组血清IL-10及IL-12水平分别为 (16.80±7.96)和 (60.18±72.59)ng/L,也都明显高于正常对照组水平 (8.45±4.56)和 (34.10±15.55)ng/L(分别P<0.01、P<0.05) ;ALT异常组血清IL -10水平与ALT正常组水平 (8.78±3.31)ng/L比较差别有显著性意义 (P<0.01)。结论 HBV慢性感染者血清IL-12、IFN -γ和IL-10水平升高可能与免疫病理性肝损伤有关
Objective To investigate the clinical significance of detecting serum interleukin-12 (IL-12), interferon-γ (IFN-γ) and interleukin-10 (IL-10) in patients with chronic hepatitis B virus infection. Methods Serum samples of 97 patients with chronic HBV infection were isolated. Among them, 41 cases had normal levels of serum ALT and 56 cases with abnormal ALT levels. Serum IL-12, IFN-γ and IL-10 levels were detected by double antibody sandwich ELISA. Results Serum levels of IFN-γ in patients with chronic HBV infection and those with ALT abnormalities were (16.62 ± 15.15) and (24.91 ± 48.68) ng / L, respectively, which were significantly higher than those in patients with normal HBV infection (6.25 ± 2.22) ng / L (P <0.01). The levels of IL-10 and IL-12 in the ALT group were significantly higher than those in the control group (16.80 ± 7.96 and 60.18 ± 72.59) ng / L 4.56) and (34.10 ± 15.55) ng / L respectively (P <0.01, P <0.05, respectively). The level of IL-10 in ALT abnormal group was significantly lower than that in normal ALT group (8.78 ± 3.31) ng / L P <0.01). Conclusion Serum levels of IL-12, IFN-γ and IL-10 in patients with chronic HBV infection may be related to immunopathological liver injury